51976

Ethyl alcohol, pure

≥99.8%, for molecular biology, (absolute alcohol, without additive, A15 o1), BioUltra

• Synonym(e): Ethylalkohol
• Lineare Formel: CH₃CH₂OH
• CAS-Nummer: 64-17-5
• Molekulargewicht: 46.07
• NACRES: NA.25
• PubChem Substance ID: 329757771
• UNSPSC Code: 12191601
• EC Number: 200-578-6
• MDL number: MFCD00003568
• Beilstein/REAXYS Number: 1718733
• Grade: Molecular Biology
• Assay: ≥99.8%
• Application(s): life science and biopharma
• Bp: 78 °C (lit.)
• Vapor pressure: 44.6 mmHg ( 20 °C)

Eigenschaften

• Produktname: Ethanol, BioUltra, Molecular Biology, ≥99.8%, (absolute alcohol, without additive, A15 o¹)
• InChI key: LFQSCWFLJHTTHZ-UHFFFAOYSA-N
• InChI: 1S/C2H6O/c1-2-3/h3H,2H2,1H3
• SMILES string: CCO
• grade: Molecular Biology
• vapor density: 1.59 (vs air)
• vapor pressure: 44.6 mmHg ( 20 °C)
• product line: BioUltra
• assay: ≥99.8%
• form: liquid
• autoignition temp.: 683 °F
• quality: (absolute alcohol, without additive, A15 o¹)
• expl. lim.: 19 %, 60 °F
• impurities: DNases, none detected; KMnO₄-reducing substances, in accordance; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; substances darkened by H₂SO₄, in accordance; ≤0.0004% free alkali (as NH₃); ≤0.001% aldehyde (as CH₃CHO); ≤0.001% formaldehyde (as HCHO); ≤0.001% ketone (as CH₃COCH₃); ≤0.002% free acid (as CH₃COOH); ≤0.1% methanol (CH₃OH); ≤0.2% water
• evapn. residue: ≤0.001%
• refractive index: n20/D 1.3600 (lit.); n20/D 1.362
• bp: 78 °C (lit.)
• mp: −114 °C (lit.)
• solubility: H₂O: miscible; organic solvents: miscible
• cation traces: Al: ≤0.5 mg/kg; Ba: ≤0.1 mg/kg; Bi: ≤0.1 mg/kg; Ca: ≤0.5 mg/kg; Cd: ≤0.05 mg/kg; Co: ≤0.02 mg/kg; Cr: ≤0.02 mg/kg; Cu: ≤0.1 mg/kg; Fe: ≤0.1 mg/kg; K: ≤0.5 mg/kg; Li: ≤0.1 mg/kg; Mg: ≤0.1 mg/kg; Mn: ≤0.02 mg/kg; Mo: ≤0.1 mg/kg; Na: ≤1 mg/kg; Ni: ≤0.02 mg/kg; Pb: ≤0.1 mg/kg; Sr: ≤0.1 mg/kg; Zn: ≤0.1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.04; λ: 280 nm A_max: 0.03
• suitability: suitable for molecular biology
• application(s): life science and biopharma
• format: neat
• Quality Level: 200

Beschreibung

Other Notes

¹Der Vertrieb und der Einsatz von Ethylalkohol in der Schweiz erfordert eine Lizenz der Eidgenössischen Alkoholverwaltung

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Sales restrictions may apply. Suitable for the precipitation of nucleic acids[1]

Application

Ethanol has been used:

• in vehicle-treated controls to perform mouse experiments
• in viability assay along with crystal violet to stain human and mouse high-grade serous ovarian cancer (HGSOC) cells after stimulation
• to prepare 17β-estradiol (E2) to use in cell culture experiments
• in cell cycle analysis for fixing cells drop-wise

Biochem/physiol Actions

Ethanol is a widely used organic chemical in industry and consumer goods. This aliphatic alcohol is mainly used in industry as a solvent and as an intermediate in the production of other chemicals.

Features and Benefits

• Versatile and adaptable for a wide variety of laboratory and research applications
• BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

General description

Ethanol, also known as Ethyl Alcohol, is a volatile primary alcohol with a straight-chain structure. It is frequently employed as a biochemical reagent, effectively denaturing proteins from bacteria, fungi, and viruses. Widely utilized in cell biology, molecular biology, and biochemical research, this DNase, RNase, NICKase, and protease-free form of ethanol plays a crucial role in purifying and precipitating biomolecules like nucleic acids (DNA & RNA) and proteins. Additionally, it finds application in histology for preparing staining and destaining reagents, as well as in the dehydration of tissues before embedding.

Sicherheitshinweise

• pictograms: GHS02,GHS07
• signalword: Danger
• hcodes: H225,H319
• pcodes: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2
• Lagerklasse: 3 - Flammable liquids
• wgk: WGK 1
• flash_point_f: 55.4 °F - closed cup
• flash_point_c: 13 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter