47608

Formaldehyd -Lösung

Molecular Biology, BioReagent, ≥36.0% in H₂O (T)

• Synonym(e): Formalin
• Lineare Formel: HCHO
• CAS-Nummer: 50-00-0
• Molekulargewicht: 30.03
• UNSPSC Code: 12352114
• NACRES: NA.52
• PubChem Substance ID: 329757328
• MDL number: MFCD00003274
• Beilstein/REAXYS Number: 1209228
• Grade: Molecular Biology
• Technique(s): electrophoresis: suitable
• Vapor pressure: 52 mmHg ( 37 °C)

Eigenschaften

• Produktname: Formaldehyd -Lösung, Molecular Biology, BioReagent, ≥36.0% in H₂O (T)
• InChI: 1S/CH2O/c1-2/h1H2
• SMILES string: [H]C([H])=O
• InChI key: WSFSSNUMVMOOMR-UHFFFAOYSA-N
• grade: Molecular Biology
• vapor density: 1.03 (vs air)
• vapor pressure: 52 mmHg ( 37 °C)
• product line: BioReagent
• form: liquid
• autoignition temp.: 572 °F
• contains: ~10% methanol as stabilizer
• concentration: ≥36.0% in H₂O (T); 36.0-38.0% (aldehyde method)
• technique(s): electrophoresis: suitable
• impurities: ≤0.03% free acid (as HCOOH)
• ign. residue: ≤0.05% (as SO₄)
• pH: 2.8-4.0
• density: 1.09 g/mL at 20 °C; 1.09 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤10 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• cation traces: Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Na: ≤100 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Zn: ≤1 mg/mL
• λ: 2 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.01; λ: 280 nm A_max: 0.01
• suitability: in accordance for electrophoresis of RNA in agarose gels
• storage temp.: room temp
• Quality Level: 100

Beschreibung

Disclaimer

kann Niederschlag enthalten (Paraformaldehyd)

Application

Formaldehyde solution is suitable for:

• denaturation and separation of RNA molecules
• anti-bacterial treatment
• fixation of cells

Formaldehydesolution has been used:

• in the fixation of human spermatogonial stem cell transplantation (SSCT).[1]
• to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.[2]
• in fixing zebra fish larvae for neutrophil staining.[3]

Biochem/physiol Actions

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

General description

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.[4]

Sicherheitshinweise

• pictograms: GHS02,GHS06,GHS08,GHS05
• signalword: Danger
• hcodes: H226,H301 + H311,H314,H317,H330,H335,H341,H350,H370
• pcodes: P202 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
• target_organs: Eyes,Central nervous system, Respiratory system
• Lagerklasse: 3 - Flammable liquids
• wgk: WGK 3
• flash_point_f: 133.0 °F - closed cup
• flash_point_c: 56.11 °C - closed cup
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter