The Chromolith® columns do not contain silica particles but are made of monolithic silica, which is a bimodal, porous, silica rod with pore sizes of 2 microns and 13 nanometers. This combination dramatically reduces separation time while increasing efficiency.
1.52022
Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns
L × I.D. 100 mm x 4.6 mm HPLC Column
Synonyme(s) :
Chromolith® HighResolution RP-18 endcapped 100-4.6 HPLC column
Se connecter pour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Sélectionner une taille de conditionnement
Le tarif et la disponibilité ne sont pas disponibles actuellement.
A propos de cet article
Code UNSPSC :
41115709
Nomenclature NACRES :
SB.52
Passer à
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderNom du produit
Chromolith® HighResolution RP-18 endcapped, L × I.D. 100 mm x 4.6 mm HPLC column
Matériaux
PEEK column
Niveau de qualité
Agence
suitable for USP L1
Gamme de produits
Chromolith®
Caractéristiques
endcapped
endcapped
Conditionnement
1 ea of
Ampleur du marquage
15% Carbon loading
18% Carbon loading
Paramètres
200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.
Technique(s)
HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)
Colonne, L × D.I.
10 cm × 4.6 mm
Superficie
250 m2/g × 250 m2/g
Matrice
highly porous
monolithic silica
monolithic silica
Groupe de la matrice active
C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase
Dimension de pores
1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity
Plage de pH de fonctionnement
2-7.5
2-7.5
Application(s)
PFAS testing
Technique de séparation
reversed phase
Température de stockage
no temp limit
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
1 of 4
Cet article | 1.52020 | 1.52023 | 1.52021 |
|---|---|---|---|
| matrix active group C18 bonding phase, octadecylsilane (ODS) | matrix active group C18 bonding phase, octadecylsilane (ODS) | matrix active group C18 bonding phase, octadecylsilane (ODS) | matrix active group C18 bonding phase, octadecylsilane (ODS) |
| separation technique reversed phase | separation technique reversed phase | separation technique reversed phase | separation technique reversed phase |
| product line Chromolith® | product line Chromolith® | product line Chromolith® | product line Chromolith® |
| pore size 1.15 μm macroporosity, 15 nm mesoporosity, 1.5 μm macroporosity | pore size 1.15 μm macroporosity, 1.5 μm macroporosity, 15 nm mesoporosity | pore size 1.15 μm macroporosity, 1.5 μm macroporosity, 15 nm mesoporosity | pore size 1.15 μm macroporosity, 1.5 μm macroporosity, 15 nm mesoporosity |
| matrix highly porous, monolithic silica | matrix highly porous, monolithic silica | matrix highly porous, monolithic silica | matrix highly porous, monolithic silica |
| agency suitable for USP L1 | agency suitable for ISO/CEN 6304.09, suitable for USP L1 | agency suitable for USP L1 | agency suitable for USP L1 |
Description générale
Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for high resolution separations of complex compound mixtures on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for high resolution separations of complex compound mixtures on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns
Application
- A Novel and Sensitive LC-MS/MS Method for the Quantitation of Ceftiofur in Pharmaceutical Preparations and Milk Samples.: This study, conducted by Levent et al., introduces a highly sensitive LC-MS/MS method for quantifying ceftiofur in pharmaceutical preparations and milk samples. The method utilizes Chromolith® HighResolution RP-18 endcapped columns, showcasing their application in enhancing the resolution and sensitivity of chromatographic analysis in complex matrices (Levent et al., 2021).
Remarque sur l'analyse
Appearance: passes test
Pressure: 40 - 70 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 140000
Capacity factor (Anthracene): 3.9 - 4.6
Tailing (USP): 0.90 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35
Pressure: 40 - 70 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 140000
Capacity factor (Anthracene): 3.9 - 4.6
Tailing (USP): 0.90 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35
Informations légales
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
Produit(s) apparenté(s)
Code de la classe de stockage
11 - Combustible Solids
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Protocoles
Folic acid vs. folate differences and biological activities; implications in fortified foods and supplements for human health.
Contenu apparenté
Les petites molécules sont des ions et des composés dont le poids moléculaire est généralement inférieur à 900 daltons. Ces composés peuvent être efficacement séparés et analysés par HPLC, UHPLC et LC-MS en utilisant principalement des particules de silice ou des phases stationnaires monolithiques avec une large gamme de chimies de colonne (modifications).
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
United States Pharmacopeia Methods - Acetaminophen, Aspirin and Caffeine
-
what is the particle size of Chromolith® HighResolution RP-18 endcapped column Ord No.: 1.52022.0001
1 réponse-
Utile ?
-
Filtres actifs
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique