537059

Propidium Iodide

Membrane impermeable DNA intercalator.

• Synonym(s): Propidium Iodide
• Empirical Formula (Hill Notation): C₂₇H₃₄I₂N₄
• CAS Number: 25535-16-4
• Molecular Weight: 668.39
• Numéro MDL: MFCD00011921

Properties

• Niveau de qualité: 100
• Forme: solid
• manufacturer/tradename: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: dark red
• input: sample type intact cells
• Fluorescence: λ_ex ~536 nm; λ_em ~617 nm
• Expédié(e)(s) dans: ambient
• Temp. de stockage: 2-8°C
• InChI: 1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
• InChI key: XJMOSONTPMZWPB-UHFFFAOYSA-M

Description

Description générale

Extinction coefficient(493 nm): 5900 M ^-1cm^-1;(287 nm): 55,700 M ^-1cm^-1;

Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).

Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M^-1cm^-1 (493 nm); 55,700 M^-1cm^-1 (287 nm); 13,800 M^-1cm^-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.

Conditionnement

50 , 100 mg in Glass bottle

250 mg in Plastic ampoule

1 g in Plastic ampoule

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Membrane impermeable DNA intercalator

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Irritant (B)

Notes préparatoires

Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H341
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Muta. 2
• Code de la classe de stockage: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Point d'éclair C: Not applicable