Extending the primary incubation step beyond the specified time in the kit protocol is not something that has been tested. A longer incubation time could potentially lead to decreased performance, including poor recovery or increased variability in assay characteristics. It may also result in higher nonspecific binding, leading to elevated signals in background wells and a loss of sensitivity.
HTH17MAG-14K
MILLIPLEX® Human Th17 Panel
Configurable Human Th17 25-Plex Panel
Synonyme(s) :
Human Th17 cytokine multiplex kit, Luminex® human Th17 cytokine immunoassay, Millipore human Th17 cytokine panel
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A propos de cet article
UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
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Laissez-nous vous aiderNom du produit
MILLIPLEX® Human Th17 Panel, Configurable Human Th17 25-Plex Panel
species reactivity
human
Quality Level
packaging
pkg of 1 ea
manufacturer/tradename
Milliplex®
assay range
accuracy: 79-107%
standard curve range: 1-5000 pg/mL
(IL-10)
standard curve range: 10-40,000 pg/mL
(IFNγ)
standard curve range: 12-50,000 pg/mL
(IL-17A & IL-2)
standard curve range: 24-100,000 pg/mL
(IL-17 & IL-4)
standard curve range: 3-10,000 pg/mL
(IL-6 & TNFα)
standard curve range: 336-1,500,000 pg/mL
(IL-23)
standard curve range: 37-150,000 pg/mL
(IL-22 & TNFβ)
standard curve range: 488-2,000,000 pg/mL
(IL-17E/IL-25)
standard curve range: 49-200,000 pg/mL
(IL-28a & IL-31)
standard curve range: 5-20,000 pg/mL
(MIP-3α, IL-1β, IL-12 (p70), IL-15, IL-21 & IL-33)
standard curve range: 6-25,000 pg/mL
(IL-5)
standard curve range: 61-250,000 pg/mL
(GM-CSF & IL-27)
standard curve range: 7-30,000 pg/mL
(IL-13)
standard curve range: 9-35,000 pg/mL
(IL-9)
technique(s)
multiplexing: suitable
input
cell culture supernatant
serum
detection method
fluorometric (Luminex® xMAP® technology)
shipped in
wet ice
storage temp.
2-8°C
Catégories apparentées
1 of 4
Cet article | HT17MG-14K-PX25 | MT17MAG47K-PX25 | HCD8MAG-15K |
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity mouse | species reactivity human |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| assay range accuracy: 79-107%, standard curve range: 10-40,000 pg/mL | assay range accuracy: 79-107%, standard curve range: 1-5000 pg/mL | assay range accuracy: 85-110%, linearity: 111.0% | assay range - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) |
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 25 analytes in human serum, plasma, and cell culture supernatants.Analytes included: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβAssay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Disclaimer
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
Features and Benefits
Comprehensive 25-Plex Panel: Simultaneously quantifies 25 Th17-related cytokines and chemokines including IL-17A, IL-17F, IL-21, IL-22, IL-23, TNF-α, and more.Multiplexing with Luminex® xMAP Technology: Enables high-throughput analysis of multiple biomarkers in a single 25 µL sample, saving time and resources.Verified for Multiple Sample Types: Compatible with human serum, plasma, and cell culture supernatants, offering flexibility for various research models.Optimized Serum Matrix: Mimics native analyte environments for more physiologically relevant results.Supports Autoimmune and Cancer Research: Ideal for studying Th17 involvement in diseases like multiple sclerosis, rheumatoid arthritis, Crohn′s disease, and tumor progression.
General description
CD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFNγ, secrete IFNγ that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation. Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn′s disease, and autoimmune uveitis. In addition, Th17 cells play an important role in tumor development, progression, and metastasis, potentially in both promoting and inhibiting tumor growth.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
target_organs
Respiratory Tract
Classe de stockage
10 - Combustible liquids
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Contenu apparenté
Immunology multiplex assays, like MILLIPLEX® assays, allow researchers to investigate more immune biomarkers simultaneously, saving time and resources.
Discover the benefits of MILLIPLEX® multiplex assays, based on Luminex® xMAP® multiplex assay technology, that provide consistent, high-quality results and see how these multiplex biomarker Luminex® assays are being used to advance research.
See how multiplexing the inflammation signaling pathway with MILLIPLEX® inflammation assays or cell signaling assays can help researchers bridge the gap between immunology and cell signaling, including investigating T cell signaling, Th Cell differentiation, inflammatory response signaling, and sepsis signaling.
MILLIPLEX® MAP. Bring your biomarkers to life. The best, most relevant multiplex assays for neuroscience and immunology research. Research points to inflammation playing a role in many neurological disorders, such as Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Since no single biomarker can tell you all you need to discover the role of inflammation in neurological disorders, EMD Millipore enables you to measure multiple circulating or intracellular biomarkers related to neuroinflammation in a single, small sample, using our extensive list of multiplex assay panels. MILLIPLEX® map multiplex assays give you a head start on your research by enabling you to work faster and smarter than with traditional techniques, saving sample, time, labor and cost with our consistent, high-quality assays.
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Could you please provide information on the potential effects of leaving samples on the shaker overnight for longer than 18 hours, for example, 24-30 hours instead of the recommended 16-18 hours?
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