LSKMAGS15
Support magnétique PureProteome 15 ml
The PureProteome Magnetic Stand, 15 mL is designed for use with PureProteome Magnetic Beads in affinity purifications (e. g., His-tag purifications or immunoprecipitations).
Synonyme(s) :
Support magnétique pour les billes magnétiques PureProteome, Support magnétique pour les tubes de 15 ml
Sélectionner une taille de conditionnement
A propos de cet article
Passer à
Matériaux
self-standing
Caractéristiques
binder
Fabricant/nom de marque
PureProteome
Technique(s)
RNA purification: suitable (with magnetic beads)
protein purification: suitable
Conditions d'expédition
ambient
Catégories apparentées
1 of 4
Cet article | LSKMAGL10 | LSKMAGS08 | LSKMAGG |
|---|---|---|---|
| feature binder | feature - | feature binder | feature - |
| manufacturer/tradename PureProteome | manufacturer/tradename PureProteome | manufacturer/tradename PureProteome | manufacturer/tradename PureProteome |
| material self-standing | material - | material self-standing | material - |
| technique(s) RNA purification: suitable (with magnetic beads) | technique(s) depletion: suitable (serum), protein purification: suitable | technique(s) RNA purification: suitable (with magnetic beads), protein purification: suitable | technique(s) depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable |
| shipped in ambient | shipped in wet ice | shipped in ambient | shipped in wet ice |
Description générale
Application
- purification à l'aide de billes magnétiques
- purification des protéines
- purification du complexe protéique impliquant la protéine de choc thermique 90 (Hsp90)/Cdc37 (cycle de division cellulaire 37)/kinase dépendante des cyclines 4 (Cdk4), pour incuber et laver les billes avec 10 volumes de tampon de lyse[1]
Autres remarques
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Contenu apparenté
Read an automated protocol for protein purification using PureProteome™ nickel magnetic beads on the AAW™ automated assay workstation and see results comparing manual vs automated runs.
Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.
Filtres actifs
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique