If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
83264
HEPES solution
1 M in H₂O, liquid
Synonyme(s) :
Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique)
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94,80 €
344,00 €
A propos de cet article
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Nom du produit
HEPES solution, 1 M in H2O
SMILES string
OCCN1CCN(CCS(=O)(O)=O)CC1
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
form
liquid
concentration
1 M in H2O
238 g/L HEPES in H2O
impurities
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
pH
5.5±0.5
useful pH range
6.8-8.2
density
1.07 g/mL at 20 °C
application(s)
diagnostic assay manufacturing
Quality Level
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Catégories apparentées
1 of 4
Cet article | H0887 | H0887 | H7523 |
|---|---|---|---|
| concentration 1 M in H2O, 238 g/L HEPES in H2O | concentration 1 M | concentration 1 M | concentration - |
| Quality Level 100 | Quality Level 300 | Quality Level 300 | Quality Level 400 |
| form liquid | form liquid | form liquid | form powder |
| application(s) diagnostic assay manufacturing | application(s) cell analysis | application(s) cell analysis | application(s) diagnostic assay manufacturing |
| density 1.07 g/mL at 20 °C | density 1.1 g/cm3 | density 1.1 g/cm3 | density - |
| pH 5.5±0.5 | pH 7.0-7.6 | pH 7.0-7.6 | pH 5.0-6.5 (1 M in H2O) |
| concentration 1 M in H2O, 238 g/L HEPES in H2O | concentration 1 M | concentration 1 M | concentration - |
| Quality Level 100 | Quality Level 300 | Quality Level 300 | Quality Level 400 |
| form liquid | form liquid | form liquid | form powder |
| application(s) diagnostic assay manufacturing | application(s) cell analysis | application(s) cell analysis | application(s) diagnostic assay manufacturing |
| density 1.07 g/mL at 20 °C | density 1.1 g/cm3 | density 1.1 g/cm3 | density - |
| pH 5.5±0.5 | pH 7.0-7.6 | pH 7.0-7.6 | pH 5.0-6.5 (1 M in H2O) |
Application
- As a component of SAPI medium used for the culturing of S. Typhymurium inoculum[1]
- To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI[2][3]
- As a component of dissection buffer for culturing rat cortical neurons[4]
- Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses[5]
- As a component of Danieu′s aquarium water used in agarose gel preparation[6]
General description
Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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How can I determine the shelf life / expiration / retest date of this product?
1 réponse-
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 réponse-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can the pH be raised from 5.5 to 8.3?
1 réponse-
To increase the pH from 5.5 to 8.3, you can use 0.1 M NaOH.
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해당 HEPES buffer solution 에 HEPES 분자 외에 다른 salt 들 (ex. Na, K 등) 은 없는 상태인가요? 단순히 물에 HEPES 분자가 풀려있는 상황인지 궁금합니다.
1 réponse-
This product is comprised only of HEPES in water. There are no other salts or additives present in the material.
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