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Merck

A5598

Anti-c-Myc−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-c-Myc antibody produced in rabbit

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:

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Nom du produit

Anti-c-Myc−Peroxidase antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

1.8-4 mg/mL

technique(s)

western blot: 1:5,000

UniProt accession no.

storage temp.

−20°C

Quality Level

Gene Information

human ... MYC(4609)

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Cet article
C3956SAB4503660B7554
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

unconjugated

conjugate

biotin conjugate

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

9E10, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human, mouse, rat

species reactivity

human

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

technique(s)

western blot: 1:5,000

technique(s)

immunocytochemistry: 5-10 μg/mL, immunoprecipitation (IP): 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts, indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells, microarray: suitable, western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates.

technique(s)

ELISA: 1:40000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein

Application

Antibody is suitable for immunoblotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

General description

The Anti-c-Myc peroxidase conjugate rabbit antibody reacts with c-Myc tagged fusion proteins. It recognizes the c-Myc tag sequence expressed at either the amino- or the carboxyl-terminus.

Immunogen

peptide corresponding amino acids 408-425 of the human c-myc proto-oncogene, conjugated to maleimide-activated KLH through a C-terminal added cysteine residue.

Physical form

Solution in 0.01 M sodium phosphate buffer saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

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Warning

hcodes

Hazard Classifications

Skin Sens. 1

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Yang Lu et al.
Molecular biology of the cell, 19(10), 4260-4272 (2008-08-08)
Efg1 is essential for hyphal development and virulence in the human pathogenic fungus Candida albicans. How Efg1 regulates gene expression is unknown. Here, we show that Efg1 interacts with components of the nucleosome acetyltransferase of H4 (NuA4) histone acetyltransferase (HAT)
Xiang He et al.
PLoS pathogens, 13(6), e1006347-e1006347 (2017-06-08)
Estrogen-related receptor α (ERRα) is a member of the nuclear receptor superfamily controlling energy homeostasis; however, its precise role in regulating antiviral innate immunity remains to be clarified. Here, we showed that ERRα deficiency conferred resistance to viral infection both
Camilla Kreßner et al.
PloS one, 8(8), e69947-e69947 (2013-08-13)
We have recently characterized SCAI (Suppressor of Cancer Cell Invasion), a transcriptional modulator regulating cancer cell motility through suppression of MAL/SRF dependent gene transcription. We show here that SCAI is expressed in a wide range of normal human tissues and
Du-Hwa Lee et al.
The Plant cell, 29(11), 2895-2920 (2017-11-01)
Dynamic control of protein translation in response to the environment is essential for the survival of plant cells. Target of rapamycin (TOR) coordinates protein synthesis with cellular energy/nutrient availability through transcriptional modulation and phosphorylation of the translation machinery. However, mechanisms
Gang Li et al.
Nature cell biology, 13(5), 616-622 (2011-04-19)
The circadian clock controls many metabolic, developmental and physiological processes in a time-of-day-specific manner in both plants and animals. The photoreceptors involved in the perception of light and entrainment of the circadian clock have been well characterized in plants. However

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