Merck
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L9393

Sigma-Aldrich

Anti-Laminin antibody produced in rabbit

enhanced validation

0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s):
Laminin Antibody
Numéro MDL:
NACRES:
NA.41

Niveau de qualité

300

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

antibody product type

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

species reactivity

human, animal

Validation améliorée

independent
Learn more about Antibody Enhanced Validation

Concentration

0.5 mg/mL

technique(s)

dot blot: 1:1,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:30 using human and animal tissues
microarray: suitable

N° d'accession UniProt

application(s)

research pathology

Expédié(e)(s) dans

dry ice

Temp. de stockage

−20°C

Gene Information

human ... LAMA1(284217)
mouse ... Lama1(16772)

Description générale

Laminin is a ubiquitous non-collagenous connective tissue glycoprotein that is a major constituent of basement membranes. Laminins are extracellular matrix proteins. The LAMA1 (laminin subunit α 1) gene is mapped to human chromosome 18p11. The antibody is isolated from antiserum by immunospecific methods of purification. Antigen specific isolation removes essentially all rabbit serum proteins, including immunoglobulins that do not specifically bind to laminin.

Spécificité

Specificity of the anti-laminin antibodies is determined by indirect immunofluorescent labeling of formalin-fixed, paraffin-embedded human or animal tissue sections, and by dot blot immunoassay. By indirect immunofluorescence the antibody demonstrates specific basement membrane staining of enzymatically unmasked human and animal tissue. In the dot blot immunoassay the rabbit anti-laminin antibody reacts with laminin but not with fibronectin, vitronectin, collagen IV, or chondroitin sulfate types A, B, and C. The affinity isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources.

Immunogène

Laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.

Application

Anti-Laminin antibody has been used in immunohistochemical staining and immunohistochemistry.
Rabbit Anti-Laminin antibody may be used in immunohistochemistry for marking blood vessel walls in different species, classification of various disease processes involving basement membranes, identification of the origin of human tumors and their classification, and for distinguishing between non-invasive and invasive lesions. The antibody may be used to monitor levels of laminin in biological fluids and for experimental production of basement membrane lesions in vivo.
A working dilution of at least 1:1,000 was determined by a dot blot immunoassay using laminin at 50 ng per dot.
A working dilution of at least 1:25 was determined by indirect immunohistology using formalin-fixed, paraffin-embedded human and animal tissues.

Actions biochimiques/physiologiques

LAMA1 (laminin subunit α 1) is involved in several diseases like cancer, infections, inflammatory diseases and autoimmune disorders. Mutations in LAMA1 result in cerebellar dysplasia and cysts with and without retinal dystrophy. LAMA1 is also essential in the assembly of basement membrane and early growth of embryo.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse

Saisir un numéro de lot pour rechercher un certificat d'analyse (COA).

Certificat d'origine

Saisir un numéro de lot pour rechercher un certificat d'origine (COO).

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the protein concentration of Anti-Laminin antibody, Product L9393?

    The protein concentration is Lot/Batch dependent and is located on the Certificate of Analysis.

  4. What is the species reactivity of Anti-Laminin antibody, Product L9393?

    This affinity-isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources. This information is located on the product data sheet.

  5. What applications is Anti-Laminin antibody, Product L9393, suitable for?

    Rabbit Anti-Laminin may be used in immunohistochemistry, immunofluorescence, and dot blot analysis.  This information is located on the product datasheet available on our website.

  6. Can Anti-Laminin antibody, Product L9393, be used in Western blot analysis?

    The following reference uses this antibody for Western blot at 1:1000.  DiProspero, N.A., et al., Inflammatory Cytokines Interact to Modulate Extracellular Matrix and Astrocytic Support of Neurite Outgrowth. Exp. Neurol., 148, 628-639 (1997).

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Yongchol Shin et al.
American journal of physiology. Cell physiology, 317(2), C314-C325 (2019-06-13)
Angiogenesis is a process through which new blood vessels are formed by sprouting and elongating from existing blood vessels. Several methods have been used to replicate angiogenesis in vitro, including culturing vascular endothelial cells on Matrigel and coculturing with endothelial
Autoimmunity against laminins.
Florea F, et al.
Clinical Immunology (Orlando, Fla.), 39-52 (2016)
Mutations in LAMA1 Cause Cerebellar Dysplasia and Cysts with and without Retinal Dystrophy
Kimberly AA, et al.
American Journal of Human Genetics, 95(2), 227-234 (2014)
Solving an enigma: Arterial pole development in the zebrafish heart
Adrian CG, et al.
Developmental Biology, 290(2), 265-276 (2006)
Zui Zhang et al.
Nature communications, 10(1), 3561-3561 (2019-08-10)
Protein corona presents a major obstacle to bench-to-bedside translation of targeted drug delivery systems, severely affecting targeting yields and directing unfavorable biodistribution. Corona-mediated targeting provides a new impetus for specific drug delivery by precisely manipulating interaction modes of functional plasma

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