Accéder au contenu
Merck

SRP6053

ProBDNF human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonyme(s) :

Abrineurin, BDNF, Brain-derived neurotrophic factor

Slide 1 of 1
Se connecter pour consulter les tarifs organisationnels et contractuels.

A propos de cet article

NACRES:
NA.32
UNSPSC Code:
12352202

Principaux documents

Voir toute la documentation

Passer à

Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

ProBDNF human, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

~52 kDa

packaging

pkg of 10 μg

NCBI accession no.

P23560

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... BDNF(627)

Catégories apparentées

Comparer avec des articles similaires

Voir la comparaison complète

Montrer les différences

1 of 4

Cet article
SRP3014B3795GF301
ProBDNF human recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Sigma-Aldrich

SRP6053

ProBDNF human

BDNF human Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture

Sigma-Aldrich

SRP3014

BDNF human

Brain-derived neurotrophic factor human BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

B3795

Brain-derived neurotrophic factor human

Protéine BDNF recombinante humaine sans origine animale The BDNF protein is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation & survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system.

Sigma-Aldrich

GF301

Protéine BDNF recombinante humaine sans origine animale

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

>98% (SDS-PAGE and HPLC)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

biological source

human

biological source

human

biological source

human

biological source

-

mol wt

~52 kDa

mol wt

27.0 kDa

mol wt

~27 kDa

mol wt

-

form

lyophilized

form

lyophilized

form

lyophilized powder

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

Biochem/physiol Actions

Precursor form of brain-derived neurotrophic factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) (2) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the tropomyosin-related kinase B (TrkB) kinase receptor and promotes the survival, growth and differentiation of neurons.[1] As proneurotrophins and mature neurotrophins elicit opposite biological effects, pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.

General description

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors.[1][2] The precursor form of brain-derived neurotrophic factor (pro-BDNF) undergoes post-translational cleavage to form mature BDNF (mBDNF). The gene encoding this protein is localized on human chromosome 11p14.1.[3][4]

Other Notes

Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu.

Physical form

Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.

Preparation Note

Centrifuge the vial prior to opening.
Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
7K014273
7K194273

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

The presence of a single-nucleotide polymorphism in the BDNF gene affects the rate of locomotor adaptation after stroke.
Helm EE
Experimental Brain Research. Experimentelle Hirnforschung. Experimentation Cerebrale, 234(2), 341-351 (2016)
11p14.1 microdeletions associated with ADHD, autism, developmental delay, and obesity.
Shinawi M
American Journal of Medical Genetics. Part A, 155A(6), 1272-1280 (2011)
Function and evolution in the NGF family and its receptors.
Ebendal T
Journal of Neuroscience Research, 32(4), 461-470 (1992)
Hao-Yun Zhang et al.
Biochimica et biophysica acta, 1843(12), 2967-2975 (2014-09-23)
Iron accumulation is observed in the substantia nigra of patients with Parkinson's disease. However, it is unknown whether neurotrophic factors, brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) participate in the modulation of neuronal iron metabolism. Here
Guoqi Zhu et al.
British journal of pharmacology, 172(9), 2354-2368 (2015-01-07)
Mild cognitive deficit in early Parkinson's disease (PD) has been widely studied. Here we have examined the effects of memantine in preventing memory deficit in experimental PD models and elucidated some of the underlying mechanisms. I.p. injection of 1-methyl-4- phenyl-1,2,3,6-tetrahydro
Afficher tous les articles scientifiques apparentés

Questions

  1. Hi, Is this recombinant protein functional?

    1 réponse

    1. This product is a growth factor analyzed for Appearance, Purity, and Endotoxin level. Kindly review the following citation where this material was used in Primary rat VM neuron culture.

      Zhang HY, Song N, Jiang H, Bi MX, Xie JX. Brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor inhibit ferrous iron influx via divalent metal transporter 1 and iron regulatory protein 1 regulation in ventral mesencephalic neurons. Biochim Biophys Acta. 2014 Dec;1843(12):2967-75. doi: 10.1016/j.bbamcr.2014.09.010. Epub 2014 Sep 16. PMID: 25239763.

      Utile ?

Évaluations

Aucune valeur de notation

Filtres actifs

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique