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Merck

M6250

2-mercaptoetanolo

≥99.0%

Sinonimo/i:

β-mercaptoetanolo, 2-idrossietilmercaptano, BME, Thioethylene glycol

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10 ML

48,30 €

100 ML

56,40 €

250 ML

87,70 €

500 ML

114,00 €

1 L

192,95 €

48,30 €

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Informazioni su questo articolo

Formula condensata:
HSCH2CH2OH
Numero CAS:
60-24-2
Peso molecolare:
78.13
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
57654402
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
MFCD00004890
Assay:
≥99.0%
Bp:
157 °C (lit.)
Vapor pressure:
1 mmHg ( 20 °C)

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Nome del prodotto

2-mercaptoetanolo, ≥99.0%

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

SMILES string

OCCS

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

assay

≥99.0%

form

liquid

expl. lim.

18 %

concentration

14.3 M (pure liquid)

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

storage temp.

2-8°C

Quality Level

200

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Questo articolo
9762213715163689
2-mercaptoetanolo ≥99.0%

Sigma-Aldrich

M6250

2-mercaptoetanolo

2-mercaptoetanolo derivatization grade (HPLC), LiChropur™, ≥99.0% (GC)

Supelco

97622

2-mercaptoetanolo

2-mercaptoetanolo EMPROVE® EVOLVE

SAFC

137151

2-mercaptoetanolo

2-mercaptoetanolo BioUltra, Molecular Biology, ≥99.0% (GC)

Sigma-Aldrich

63689

2-mercaptoetanolo

assay

≥99.0%

assay

≥99.0% (GC)

assay

-

assay

≥99.0% (GC)

Quality Level

200

Quality Level

100

Quality Level

400

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

refractive index

n20/D 1.500 (lit.)

refractive index

n20/D 1.500 (lit.), n20/D 1.500-1.502

refractive index

n20/D 1.500 (lit.)

refractive index

n20/D 1.500 (lit.), n20/D 1.501

density

1.114 g/mL at 25 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

expl. lim.

18 %

expl. lim.

18 %

expl. lim.

18 %

expl. lim.

18 %

Application

Il 2-mercaptoetanolo è impiegato diffusamente per ritardare l′ossidazione dei composti biologici in soluzione.
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

General description

Il 2-mercaptoetanolo è un composto tiolico[1] usato comunemente come agente riducente nelle reazioni organiche.[2]

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signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
SHBT4347
BCCP0675
BCCM9818
SHBS4916
BCCM8003

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Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines.
Asfari M, et al.
Endocrinology, 130(1), 167-178 (1992)
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
Morrissey JH.
Analytical Biochemistry, 117(2), 307-310 (1981)
Katharina Rothe et al.
Blood, 123(23), 3622-3634 (2014-04-24)
Previous studies demonstrated that imatinib mesylate (IM) induces autophagy in chronic myeloid leukemia (CML) and that this process is critical to cell survival upon therapy. However, it is not known if the autophagic process differs at basal levels between CML
Manching Ku et al.
PLoS genetics, 4(10), e1000242-e1000242 (2008-11-01)
In embryonic stem (ES) cells, bivalent chromatin domains with overlapping repressive (H3 lysine 27 tri-methylation) and activating (H3 lysine 4 tri-methylation) histone modifications mark the promoters of more than 2,000 genes. To gain insight into the structure and function of
Emily Cunningham Williams et al.
Human molecular genetics, 23(11), 2968-2980 (2014-01-15)
The disease mechanism of Rett syndrome (RTT) is not well understood. Studies in RTT mouse models have suggested a non-cell-autonomous role for astrocytes in RTT pathogenesis. However, it is not clear whether this is also true for human RTT astrocytes.
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Domande

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Utile?

  2. Can this bME be use in cell culture?

    1 risposta

    1. This product has not been assessed for suitability in cell culture. Alternative product M3148, 2-Mercaptoethanol, is labeled as suitable for cell culture.

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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