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Merck

63689

2-mercaptoetanolo

BioUltra, Molecular Biology, ≥99.0% (GC)

Sinonimo/i:

β-mercaptoetanolo, 2-idrossietilmercaptano, BME, Thioethylene glycol

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25 ML

32,00 €

100 ML

96,30 €

32,00 €

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Informazioni su questo articolo

Formula condensata:
HSCH2CH2OH
Numero CAS:
60-24-2
Peso molecolare:
78.13
UNSPSC Code:
12161504
NACRES:
NA.25
PubChem Substance ID:
57651953
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
MFCD00004890

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Nome del prodotto

2-mercaptoetanolo, BioUltra, Molecular Biology, ≥99.0% (GC)

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

SMILES string

OCCS

grade

Molecular Biology

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioUltra

assay

≥99.0% (GC)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

concentration

14.3 M (pure liquid)

technique(s)

RNA extraction: suitable
protein extraction: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.500 (lit.)
n20/D 1.501

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.114 g/mL at 25 °C (lit.)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤1 mg/kg
Ni: ≤0.05 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 1.5
λ: 280 nm Amax: 0.3

storage temp.

2-8°C

Quality Level

200

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Questo articolo
8.0574097622M6250
2-mercaptoetanolo BioUltra, Molecular Biology, ≥99.0% (GC)

Sigma-Aldrich

63689

2-mercaptoetanolo

2-mercaptoetanolo for synthesis

Sigma-Aldrich

8.05740

2-mercaptoetanolo

2-mercaptoetanolo derivatization grade (HPLC), LiChropur™, ≥99.0% (GC)

Supelco

97622

2-mercaptoetanolo

2-mercaptoetanolo ≥99.0%

Sigma-Aldrich

M6250

2-mercaptoetanolo

technique(s)

RNA extraction: suitable, protein extraction: suitable

technique(s)

-

technique(s)

HPLC: suitable

technique(s)

-

grade

for molecular biology

grade

-

grade

for HPLC derivatization

grade

-

assay

≥99.0% (GC)

assay

≥99% (GC)

assay

≥99.0% (GC)

assay

≥99.0%

product line

BioUltra

product line

-

product line

-

product line

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

storage temp.

2-8°C

storage temp.

2-30°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

2-Mercaptoethanol has been used
  • in the protein extraction buffer prepared for leaf samples.[1]
  • as a supplement in cell culture media.[2]
  • in the procedure of RNA extraction.[3]
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds[4]

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
BCCK9816
BCCH9530
BCCH3295
BCCG5954
BCCG0639

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The interplay between protein L-isoaspartyl methyltransferase activity and insulin-like signaling to extend lifespan in Caenorhabditis elegans.
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Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells.
Benitez JJ et al.
Lab on a chip, 12, 4848-4848 (2012)
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Plant methods, 10, 21-21 (2014-07-24)
Next-generation sequencing technologies rely on high quality DNA that is suitable for library preparation followed by sequencing. Some plant species store large amounts of phenolics and polysaccharides within their leaf tissue making genomic DNA extraction difficult. While many DNA extraction
Isolation of RNA using guanidinium salts.
R J MacDonald et al.
Methods in enzymology, 152, 219-227 (1987-01-01)
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Protocolli

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Domande

1–4 di 4 domande  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Utile?

  2. Is this product suitable for cell culture?

    1 risposta

    1. This product has not been tested for use in cell culture and is listed as a molecular biology grade material. See product M3148 at the link below to review the cell culture tested product option:
      https://www.sigmaaldrich.com/product/sigma/m3148

      Utile?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

  4. How do I know when 2-Mercaptoethanol expires

    1 risposta

    1. This material is quite stable when stored and handled properly. One visual indication of degradation would be a color change to a darker yellow to brown. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

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