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14-840-M

AMPK (α1, β1, γ1) Protein, active, 10 µg

Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

Sinonimo/i:

AMPK kinase

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14-840

944,00 €

14-840M

7.200,00 €

944,00 €

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Informazioni su questo articolo

Codice UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.32

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Origine biologica

human

Livello qualitativo

100

Ricombinante

expressed in E. coli

Stato

liquid

Durata

6 mo.

PM

Mw 30 kDa (AMPKß1)
Mw 38 kDa (AMPKγ1)
Mw 64 kDa (AMPKα1)

Produttore/marchio commerciale

Upstate®

tecniche

activity assay: suitable (kinase)

Solubilità

water: soluble

N° accesso Genebanck

AF100763

N° accesso NCBI

NM_002733
NM_006253

N° accesso UniProt

P54619
Q13131
Q9Y478

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... PRKAB1(5564)

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AMPK (α1, β1, γ1) Protein, active, 10 µg Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

Sigma-Aldrich

14-840-M

AMPK (α1, β1, γ1) Protein, active, 10 µg

AMPK (α2, β1, γ1) Protein, active, 10 µg Active, recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

Sigma-Aldrich

14-902-M

AMPK (α2, β1, γ1) Protein, active, 10 µg

MKK7β1 Protein, active, 10 µg Active, N-terminal GST-tagged, recombinant, human MKK7β1, amino acids 2-end. For use in Kinase Assays.

Sigma-Aldrich

14-543-M

MKK7β1 Protein, active, 10 µg

CaM Kinase I Protein, active, 10 µg Active, N-terminal GST-tagged, recombinant, human CaMK1, amino acids 2-end. For use in Kinase Assays.

Sigma-Aldrich

14-663-M

CaM Kinase I Protein, active, 10 µg

biological source

human

biological source

human

biological source

human

biological source

human

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

mol wt

Mw 30 kDa (AMPKß1), Mw 64 kDa (AMPKα1), Mw 38 kDa (AMPKγ1)

mol wt

Mw 30 kDa (AMPKß1), Mw 38 kDa (AMPKγ1), Mw 63 kDa (AMPKα2)

mol wt

Mw 74 kDa

mol wt

Mw 68 kDa

form

liquid

form

-

form

-

form

-

UniProt accession no.

P54619, Q9Y478, Q13131

UniProt accession no.

P54619, P54646, P54646, Q9Y478, P54619, Q9Y478

UniProt accession no.

O14733

UniProt accession no.

Q14012

Descrizione generale

Research area: Cell Signaling

Product Source: Expressed in E. coli cells and activated in vivo.

Protein Target: AMPK (α1, β1, γ1)

Target Sub-Family: CAMK

Recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full- length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1. The AMPK α catalytic subunit in mammals is encoded by α1 and α2, two β genes β1 and β2 and three γ subunit genes γ1, γ2, and γ3.[1]

Applicazioni

For use in Kinase Assays.
Research Category
Metabolism

Apoptosis & Cancer
Research Sub Category
Diabetes

Obesity

Metabolic Disorders

Azioni biochim/fisiol

Under conditions of nutrient scarcity, AMPK functions as a metabolic checkpoint and inhibits cellular growth.[1] Upon activation, AMPK promotes glucose uptake and fatty acid oxidation thereby reducing energy-consuming processes like protein and lipid synthesis.[2] Furthermore, AMPK regulates autophagy and mitophagy.[3] It also facilitates catabolic pathways to generate more ATP as a response to decreased ATP production.[1]

Confezionamento

Also available in 250µg size as catalog # 14-840M - Call for pricing and availability.

Stato fisico

Cobalt-agarose

Nota sulla preparazione

6 months at -70°C

Risultati analitici

Routinely evaluated by phosphorylation of substrate peptide

Altre note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Note legali

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H317

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

AMPK: guardian of metabolism and mitochondrial homeostasis
Herzig S and Shaw RJ
Molecular Cell Biology, 19, 121?135-121?135 (2018)
AMPK and the biochemistry of exercise: Implications for human health and disease
Richter EA and Ruderman NB
The Biochemical Journal, 418(2), 261?275-261?275 (2009)
The AMP-activated protein kinase (AMPK) signaling pathway coordinates cell growth, autophagy, & metabolism
Mihaylova MM and Shaw RJ
Nature Cell Biology, 13(9), 1016?1023-1016?1023 (2011)

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