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Merck

A4718

Agarosio

Molecular Biology

Sinonimo/i:

3,6-Anhydro-α-L-galacto-β-D-galactan

Autenticati per visualizzare i prezzi organizzativi e contrattuali.

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25 G

238,00 €

100 G

749,00 €

238,00 €

Prezzo di listino280,00 €Risparmia il 15%

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Informazioni su questo articolo

Numero CAS:
UNSPSC Code:
41105317
PubChem Substance ID:
EC Number:
232-731-8
NACRES:
NA.25
MDL number:

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Nome del prodotto

Agarosio, Molecular Biology

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

biological source

algae (marine)

grade

Molecular Biology

form

powder

technique(s)

electrophoresis: suitable

EEO

≤0.05

mp

≤75 °C (3% gel)

transition temp

gel point ≤35 °C (3% gel)

foreign activity

DNase, RNase, NICKase, none detected

storage temp.

room temp

Quality Level

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Questo articolo
A2576A9539A6013
Agarosio Molecular Biology

Sigma-Aldrich

A4718

Agarosio

Agarosio Ultra-low Gelling Temperature, molecular biology grade

Sigma-Aldrich

A2576

Agarosio

Agarosio BioReagent, Molecular Biology, low EEO

Sigma-Aldrich

A9539

Agarosio

Agarosio Type I, low EEO

Sigma-Aldrich

A6013

Agarosio

EEO

≤0.05

EEO

≤0.12

EEO

0.09-0.13

EEO

0.09-0.13

form

powder

form

powder

form

powder

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

grade

for molecular biology

grade

Molecular Biology

grade

for molecular biology

grade

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

foreign activity

DNase, RNase, NICKase, none detected

foreign activity

-

foreign activity

DNase, RNase, none detected

foreign activity

-

General description

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds.[1] Agarose is highly biocompatible and possesses variable mechanical and diffusion properties.[2] Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically.[3]

Analysis Note

Segue l′elenco delle caratteristiche specificate per il nostro agarosio:
contenuto in solfati - usato come indicatore di purezza, poiché il solfato è il principale gruppo ionico presente.
forza del gel -la forza che deve essere applicata a un gel per provocarne la rottura.
punto di gel - la temperatura alla quale una soluzione acquosa di agarosio gelifica al raffreddamento La transizione dallo stato liquido a gel delle soluzioni di agarosio è soggetto a isteresi, ovvero il punto di gel non coincide con la temperatura di fusione.
Elettroendosmosi (EEO) - indica un movimento di liquido attraverso il gel. I gruppi anionici in un gel di agarosio sono fissati alla matrice e non possono muoversi, mentre i controcationi dissociabili possono migrare verso il catodo nella matrice, dando origine a EEO. Poiché il movimento elettroforetico dei biopolimeri è solitamente diretto verso l'anodo, l'EEO può disturbarne la migrazione a causa della convezione interna.

Application

Agarose has been used in the preparation of microgel slides employed in lysis and electrophoresis.[4] It has also been used in the gel electrophoresis to separate restriction enzyme digested products in catechol-O-methyltransferase (COMT) genotyping[5]
Ideal for the separation of small DNA fragments (e.g., PCR products) differing in size by as little as 2% and compares to the resolution of DNA in polyacrylamide gels. High resolution agarose is also ideal for resolving amplification fragment length polymorphisms (AmpFLP), short tandem repeats (STR) and tri- and tetranucleotide repeats.
Low ethidium bromide and SYBR Green background staining

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Certificati d'analisi (COA)

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BMC veterinary research, 14(1), 166-166 (2018-05-24)
The original article [1] contains errors in author panels and their contributions, errors in both the Methodology and the Results sections, and errors with respect to funding sources. The affected sections of the manuscript and their respective regions of corrected
Variations in catechol-O-methyltransferase gene interact with parenting to influence attention in early development
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Shamsi MB, et al.
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Agarose gel electrophoresis for the separation of DNA fragments
Lee PY, et al.
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Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques

Domande

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. Is this agarose high-resolution, and suitable for PCR applications?

    1 risposta
    1. The High Resolution Agarose, product number A4718, is an intermediate-melting agarose that offers approximately double the resolution capability compared to standard agarose. It is capable of resolving PCR products and DNA fragments ranging from 200-800 bp that differ in size by as little as 2%. This makes it ideal for separating small DNA fragments, such as PCR products, with very small size differences, offering a resolution comparable to that achieved with polyacrylamide gels.

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