A6013

Agarosio

Type I, low EEO

• Sinonimo/i: 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE
• Numero CAS: 9012-36-6
• UNSPSC Code: 41105317
• PubChem Substance ID: 24891047
• EC Number: 232-731-8
• NACRES: NA.25
• MDL number: MFCD00081294

Proprietà

• Nome del prodotto: Agarosio, Type I, low EEO
• SMILES string: O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N
• biological source: algae (Gelidium & Gracilaria)
• type: Type I
• form: powder
• technique(s): electrophoresis: suitable
• impurities: ≤10% water
• EEO: 0.09-0.13
• transition temp: gel point 36 °C ±1.5 °C (1.5% gel)
• gel strength: ≥1200 g/cm² (1% gel)
• anion traces: sulfate (SO₄^2-): ≤0.15%
• suitability: suitable for electrophoresis
• Quality Level: 200

Descrizione

Analysis Note

Segue l′elenco delle caratteristiche specificate per il nostro agarosio:
contenuto in solfati - usato come indicatore di purezza, poiché il solfato è il principale gruppo ionico presente.
forza del gel -la forza che deve essere applicata a un gel per provocarne la rottura.
punto di gel - la temperatura alla quale una soluzione acquosa di agarosio gelifica al raffreddamento La transizione dallo stato liquido a gel delle soluzioni di agarosio è soggetto a isteresi, ovvero il punto di gel non coincide con la temperatura di fusione.
Elettroendosmosi (EEO) - indica un movimento di liquido attraverso il gel. I gruppi anionici in un gel di agarosio sono fissati alla matrice e non possono muoversi, mentre i controcationi dissociabili possono migrare verso il catodo nella matrice, dando origine a EEO. Poiché il movimento elettroforetico dei biopolimeri è solitamente diretto verso l'anodo, l'EEO può disturbarne la migrazione a causa della convezione interna.

Application

Type 1 Agarose is commonly utilized for molecular biology:

• for gel electrophoresis of DNA and RNA molecules[1][2]
• for agarose overlay in cell culture, to measure antibacterial activity by radial diffusion[3]
• for agarose overlay in cell culture for radio immunodiffusion assays to quantify antigens[4]
• for agarose overlay in plaque assays to determine viral titer
• to prepare agarose hydrogel that encapsulates a viscous media for 3D cell culture[5]
• as an ingredient of human-tissue-like MRI phantoms[6]
• for diagnostic to analyze patient samples for MRSA genome using pulsed-field gel electrophoresis[2]

Features and Benefits

• Low EEO agarose is useful for routine analysis of nucleic acids
• Suitable for electrophoresis: DNase & RNase free
• Suitable for plant cell culture
• Suitable for mammalian cell culture

General description

Agarose is a polymer isolated from red seaweed, from the species Gelidium and Gracilaria, and it is commonly used for the separation of nucleic acids. Type I agarose is often utilized in molecular biology to make agarose gels for electrophoresis, a technique used to separate and analyze DNA or RNA fragments or proteins based on their size. Agarose may also be used in plant cell culture to create hydrogels, which are gel-like materials that provide a three-dimensional matrix. This provides a supportive and biocompatible environment for plant cells, this mimics physiological conditions and conducts experiments in vivo. They are versatile for various applications in plant biology, such as studying cell growth, tissue culture, and analyzing plant responses to different stimuli.

Other Notes

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Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)