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Merck

S5761

Bicarbonato di sodio

powder, BioReagent, Molecular Biology, suitable for cell culture, suitable for insect cell culture

Sodium bicarbonate

Sinonimo/i:

Idrogenocarbonato di sodio

Autenticati per visualizzare i prezzi organizzativi e contrattuali.

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500 G

63,60 €

1 KG

112,00 €

5 KG

378,00 €

63,60 €


Disponibile per la spedizione OGGIDettagli


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Informazioni su questo articolo

Formula condensata:
NaHCO3
Numero CAS:
Peso molecolare:
84.01
UNSPSC Code:
12161702
NACRES:
NA.75
PubChem Substance ID:
EC Number:
205-633-8
Beilstein/REAXYS Number:
4153970
MDL number:
Assay:
99.5-100.5%
Grade:
Molecular Biology
Form:
powder
Solubility:
water: soluble 100 mg/mL

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Nome del prodotto

Bicarbonato di sodio, powder, BioReagent, Molecular Biology, suitable for cell culture, suitable for insect cell culture

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

SMILES string

[Na+].OC([O-])=O

grade

Molecular Biology

product line

BioReagent

assay

99.5-100.5%

form

powder

quality

300

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

pH

6.8-7.4

pKa (25 °C)

(1) 6.37, (2) 10.25 (carbonic acid)

solubility

water: soluble 100 mg/mL

density

2.16 g/mL at 25 °C (lit.)

Quality Level

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Questo articolo
S8875S6014S8761
form

powder

form

powder

form

powder

form

solution (7.5%)

assay

99.5-100.5%

assay

≥99.5%

assay

≥99.7%, 99.7-100.3%

assay

-

solubility

water: soluble 100 mg/mL

solubility

-

solubility

-

solubility

-

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

400

grade

for molecular biology

grade

-

grade

ACS reagent

grade

-

pH

6.8-7.4

pH

-

pH

-

pH

7.8-8.2

Application

Il bicarbonato di sodio è stato utilizzato:
  • come componente del sistema di coltura degli embrioni bovini durante la maturazione, fertilizzazione e coltura in vitro (IVMFC)[1]
  • come componente di una miscela 1:1 di terreno Eagle modificato di Dulbecco e miscela nutritiva di Ham F-12 durante la coltura di cellule endoteliali microvascolari bovine[2]
  • come componente del terreno ITS (insulina, transferrina e selenite) utilizzato durante l′isolamento dei neuroni dopaminergici in cellule staminali embrionali umane[3]
  • come componente del terreno utilizzato per la coltura cellulare di cellule renali embrionali umane[4]
  • come componente della soluzione di scongelamento di Beltsville utilizzata per diluire lo sperma[5]

General description

Il bicarbonato di sodio trova ampio impiego come tampone fisiologico nelle applicazioni per colture cellulari. Il pKa per la reazione bicarbonato <-> acido carbonico è 6,4. Il pKa per la reazione bicarbonato <-> carbonato è 10,3. Il bicarbonato di sodio ha due pKa: 6,4 e 10,3. È adoperato diffusamente come tampone fisiologico nelle applicazioni in vitro.

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Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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E Meyer et al.
The European journal of neuroscience, 11(3), 1105-1108 (1999-04-02)
The human slow poke (hSlo) K+ channel was tagged with GFP (green fluorescent protein) at the N-terminus of its alpha-subunit. The fusion protein was expressed transiently in HEK293 cells; it formed functional voltage-gated channels as shown by whole cell patch-clamp
M Solís et al.
Research in veterinary science, 83(3), 403-409 (2007-03-21)
Porcine rubulavirus (PoRV), also known as blue eye disease (BED) of swine, causes respiratory and reproductive problems in pigs at several developmental stages. To study the effect of PoRV infection on semen production, five boars were infected with 1 x
L Keskintepe et al.
Biology of reproduction, 55(2), 333-339 (1996-08-01)
The objective was to establish an in vitro system in which bovine oocytes can be matured, fertilized, and cultured up to the blastocyst stage without support of serum, BSA, or somatic cells. Media consisted of modified tissue culture medium 199
K Okuda et al.
Biology of reproduction, 61(4), 1017-1022 (1999-09-24)
There is sufficient evidence to prove that tumor necrosis factor alpha (TNFalpha) modulates bovine corpus luteum (CL) function. Our previous study demonstrated that functional TNFalpha receptors are present on luteal cells in bovine CL throughout the estrous cycle. The purpose
Chang-Hwan Park et al.
Methods in molecular biology (Clifton, N.J.), 407, 311-322 (2008-05-06)
In this chapter, we introduce a co-culture protocol for human embryonic stem (hES) cell differentiation in which dopamine (DA) neurons with midbrain-specific markers are efficiently derived. Human ES cells on a feeder layer of stromal cells are induced to differentiate

Articoli

Nonylphenols (NP) are ubiquitous substances that have been detected in highly diverse foodstuff. As breakdown products of nonylphenol ethoxylates, NP could end up in the aquatic environment. This article presents a rapid method for NP detection in surface water by planar solid phase extraction – planar yeast estrogen screen (pSPE-pYES).

Domande

1–2 di 2 domande  
  1. What is the size of his powder?

    1 risposta
    1. The particle size of the powder has not been measured.

      Utile?

  2. What is the shelf life after delivery and opening? Is there usually an impact on quality after 2 years? The color of the medium changed and the pH decreased. Does the solubility or function of sodium bicarbonate decrease as it gets older?

    1 risposta
    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 5 years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      After opening, various factors may influence the performance of the product. Moisture uptake and cross-contamination are common issues that are dependent on product handling and storage. If the product you are using is experience stability issues before the recommended restest date, this reporting should be investigated by our Technical Service team who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Utile?

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