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Merck

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALI
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Sigma-Aldrich
Metanolo, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
Sigma-Aldrich
Tetraidrofurano, inhibitor-free, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Metanolo, ACS reagent, ≥99.8%
Sigma-Aldrich
Idrossido di ammonio, ACS reagent, 28.0-30.0% NH3 basis
Sigma-Aldrich
N,N-dimetilformammide, ACS reagent, ≥99.8%
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.9%
Sigma-Aldrich
N,N-dimetilformammide, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Dietiletere, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor
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Cloroformio, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
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Toluene, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetone, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
Tetraidrofurano, contains 200-400 ppm BHT as inhibitor, ACS reagent, ≥99.0%
Sigma-Aldrich
Metanolo, HPLC Plus, ≥99.9%
Sigma-Aldrich
Cloroformio, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
Sigma-Aldrich
Piridina, ACS reagent, ≥99.0%
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Dietiletere, suitable for HPLC, ≥99.9%, inhibitor-free
Sigma-Aldrich
Cloroformio, contains ethanol as stabilizer, ACS reagent, ≥99.8%
Sigma-Aldrich
Cloroformio, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Toluene, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
Dietiletere, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor
Sigma-Aldrich
Idrossido di ammonio, 28% NH3 in H2O, ≥99.99% trace metals basis
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2-mercaptoetanolo, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
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2-mercaptoetanolo, ≥99.0%
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Formaldeide, Molecular Biology, 36.5-38% in H2O
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Alcool benzilico, ReagentPlus®, ≥99%
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Sodio cloruro, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)
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Formaldeide, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
SAFC
Formaldeide, contains 10-15% methanol as stabilizer, 37 wt. % in H2O
Sigma-Aldrich
Sodio cloruro, 5 M in H2O, BioReagent, Molecular Biology, suitable for cell culture