This product is prepared in DMSO at a concentration of 3.125 mg/mL, which equates to approximately 5 mM. The volume of the 1 mg package is 0.320 mL. Prior to use, dilute the product 500-fold in 0.1 M phosphate buffer, pH 7.4. Other aqueous buffers such as HBSS would also be suitable.
Note that the presence of BSA and phenol red may interfere with the fluorescence.
251765
DAR-4M AM
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.
別名:
DAR-4M AM, Diaminorhodamine-4M AM
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この商品について
実験式（ヒル表記法）:
C28H31IN4O5
分子量:
630.47
NACRES:
NA.25
UNSPSC Code:
12352202
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製品名
DAR-4M AM, A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.
assay
≥98% (HPLC)
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
dark red
fluorescence
λex ~560 nm
λem ~575 nm
shipped in
ambient
storage temp.
−20°C
Quality Level
1 of 4
当該品目 | 251760 | 251515-M | 251520-M |
|---|---|---|---|
| fluorescence λex ~560 nm, λem ~575 nm | fluorescence λex ~560 nm, λem ~575 nm | fluorescence λex ~500 nm, λem ~515 nm | fluorescence λex ~500 nm, λem ~515 nm |
| color dark red | color red-violet | color pale yellow | color pale yellow |
| form solution | form solution | form solution | form solution |
| shipped in ambient | shipped in ambient | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Nitric oxide (NO) fluorescent indicator
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no
Disclaimer
Toxicity: Irritant (B)
General description
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.
Legal Information
Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Packaging
Packaged under inert gas
Physical form
In DMSO.
Preparation Note
Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.
保管分類
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F
flash_point_c
87 °C
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
-
The product is provided in solution form but no information is provided about its volume. Please provide the volume and molarity of the solution. Also what would be the recommended way of dilution - in DMSO or directly in HBSS/PBS/media?
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