CC12

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)

liquid, clone DCS-6, Calbiochem^®

• 別名: Anti-Bcl-1, Anti-PRAD-1
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: DCS-6, monoclonal
• フォーム: liquid
• 含みます: ≤0.1% sodium azide as preservative
• 交差性: human, mouse, rat
• メーカー／製品名: Calbiochem^®
• 保管条件: do not freeze
• dilution: (Flow Cytometry (5 µg/mL); Frozen Sections ; Immunoblotting (1 µg/mL); Immunofluorescence (5 µg/mL); Immunoprecipitation ; Neutralization Studies ; Paraffin Sections (5 µg/mL, heat pre-treatment required))
• アイソタイプ: IgG2a
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CCND1(595) ; mouse ... Ccnd1(12443) ; rat ... Ccnd1(58919)

詳細

詳細

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.

Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.

This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).

免疫原

Human

recombinant human cyclin D1

アプリケーション

Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)

Immunoprecipitation (see application references)

Neutralization Studies (see application references)

Paraffin Sections (5 µg/ml, heat pre-treatment required)

包装

Please refer to vial label for lot-specific concentration.

物理的形状

In 50 mM sodium phosphate buffer, 0.2% gelatin.

アナリシスノート

Negative Control
trp E (Ab-1)

Positive Control
MCF7, or A431 cells or breast carcinoma tissue

その他情報

Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.

Inaba, T., et al. 1992. Genomics13, 565.
Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Toxicity: Standard Handling (A)

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable