The treatment with acetonitrile removes the matrix effect, including the proteins, from the samples. This means that the cortisol bound to albumin and other proteins is eliminated. The assay specifically measures the free cortisol (non-bound cortisol).
HCYTA-60K
MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A - Immunology Multiplex Assay
別名:
Human cytokine multiplex kit, Luminex® human cytokine immunoassay, Millipore human cytokine panel
ログイン 組織・契約価格を表示する
この商品について
UNSPSCコード:
12161503
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
スキップする
交差性
human
品質水準
メーカー／製品名
Milliplex®
テクニック
multiplexing: suitable
入力
cell culture supernatant(s)
plasma
serum
検出方法
fluorometric (Luminex® xMAP®)
輸送温度
wet ice
保管温度
2-8°C
詳細
Cytokines, chemokines, and growth factors are key mediatorsof immune system functions capable of signaling through autocrine, paracrine,and endocrine mechanisms. Their pleiotropic immunomodulatory propertiesallow these biomolecules to react to diverse stimuli and regulate the immuneresponse, either promoting or inhibiting inflammation.
アプリケーション
- MILLIPLEX® Qualified assays undergo rigorousassay development, verification, and Quality Control testing to achieve optimalperformance.
- Simultaneously analyze up to 48 cytokine,chemokine, and growth factor biomarkers with bead-based multiplex assays usingLuminex technology, in human serum, plasma and cell culture samples.
- Available analytes: sCD40L, EGF, Eotaxin, FGF-2,FLT-3L, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1RA,IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12(p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10,MCP-1, MCP-3, M-CSF, MDC, MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES,TGFα, TNFα, TNFβ, VEGF-A
特徴および利点
- This panel is fully configurable, with options for custom bead premixing, fixed premixed and bulk premixed panels.
- Save time and resources by simultaneously analyzing up to 48 analytes of your choice in a single sample
- Requires a small sample volume (25 µL/well)
- Choose same day or overnight incubation
- Reliable and accurate performance with proven lot-to-lot consistency
- Ready-to-use reagents
- Comes with everything you need to run your assay
- Includes a Serum Matrix to mimic the native sample environment in serum and plasma samples
- Includes high and low Quality Controls
包装
96-well plate
調製ノート
Recommended storage for kit components is 2 - 8°C.
法的情報
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免責事項
Unless otherwise stated in our catalogor other company documentation accompanying the product(s), our products areintended for research use only and are not to be used for any other purpose,which includes but is not limited to, unauthorized commercial uses, in vitrodiagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumptionor application to humans or animals.
関連製品
製品番号
詳細
価格
シグナルワード
Danger
危険有害性の分類
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
ターゲットの組織
Respiratory Tract
保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
キットコンポーネントの情報を参照してください
PRTR
キットコンポーネントの情報を参照してください
消防法
キットコンポーネントの情報を参照してください
労働安全衛生法名称等を表示すべき危険物及び有害物
キットコンポーネントの情報を参照してください
労働安全衛生法名称等を通知すべき危険物及び有害物
キットコンポーネントの情報を参照してください
カルタヘナ法
キットコンポーネントの情報を参照してください
Jan Code
キットコンポーネントの情報を参照してください
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
László Gopcsa et al.
Acta microbiologica et immunologica Hungarica (2021-11-20)
Allogeneic hematopoietic stem cell transplantation (HSCT) and coronavirus disease 2019 (COVID-19) infection can both lead to severe cytokine release syndrome (sCRS) resulting in critical illness and death. In this single institution, preliminary comparative case-series study we compared clinical and laboratory
Vimvara Vacharathit et al.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 112, 227-234 (2021-09-19)
As coronavirus disease 2019 (COVID-19) rages on worldwide, there is an urgent need to characterize immune correlates of protection from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and to identify immune determinants of COVID-19 severity. This study examined the
Manuel Cabeza-Segura et al.
British journal of cancer, 127(12), 2198-2206 (2022-10-18)
Advanced gastro-oesophageal cancer (GEA) treatment has been improved by the introduction of immune checkpoint inhibitors (CPIs), yet identifying predictive biomarkers remains a priority, particularly in patients with a combined positive score (CPS) < 5, where the benefit is less clear. Our study
Carl A Pierce et al.
medRxiv : the preprint server for health sciences (2021-02-18)
COVID-19 is more benign in children compared to adults for unknown reasons. This contrasts with viruses such as influenza where disease manifestations are often more severe in children1. We hypothesized that a more robust early innate immune response to SARS-CoV-2
Patrick P G Mulder et al.
Frontiers in immunology, 11, 621222-621222 (2021-02-16)
Severe burn injury causes local and systemic immune responses that can persist up to months, and can lead to systemic inflammatory response syndrome, organ damage and long-term sequalae such as hypertrophic scarring. To prevent these pathological conditions, a better understanding
関連コンテンツ
Find immunoassays to explore neuroimmunology and neuroinflammation cytokines and neurodegenerative disease biomarkers.
サイトカインマルチプレックスアッセイにより、研究者は免疫系を容易に調べることができます。MILLIPLEX® ヒトサイトカイン／ケモカイン／成長因子パネルAおよびBは、サイトカイン検出用のマルチプレックスアッセイであり、それぞれで、48種類のアナライトの特有の組み合わせを少量のサンプル量で同時に測定できます。
MILLIPLEX®アッセイのような免疫学マルチプレックスアッセイにより、研究者は多くの免疫バイオマーカーを同時に測定することで、時間とリソースを節約することができます。これは、多くのアナライトが異なる経路で役割を果たす免疫学研究にとって、特に重要なことです。
-
When precipitating proteins (albumin and globulins) from plasma or serum samples using an Acetonitrile solution, does cortisol, which is bound to albumin & globulin, dissociate into the solution or get removed along with the protein precipitate?
1 回答-
役に立ちましたか？
-
-
Could you please provide the expected sample ranges for the Milliplex Human Cytokine Panel A (HCYTA-60K)?
1 回答-
The serum and plasma sample values for the HCYTA-60K kit are provided. It's important to note that these values should be used as a guideline only. This data is based on a small sample size without effort for randomization and is not a true reference range.
役に立ちましたか？
-
-
Could you provide a recommendation for sample preparation for placenta and HCYTA-60K assay?
1 回答-
The Milliplex assays have not been tested or verified for use with placenta tissue homogenates. However, a reference is available using placenta homogenates in the Human Cytokine Panel 1 (HCYTOMAG-60K).
The samples were prepared as follows:
Placental homogenate: Fragments of frozen placental tissue were transferred to propylene tubes containing lysis buffer and a cocktail of protease inhibitors. The placenta samples were homogenized using an electric homogenizer on ice. After homogenization, the homogenates were centrifuged, and the protein concentration in the supernatant was measured and stored for further analysis.Reference: Molás RB, Ribeiro MR, Ramalho Dos Santos MJC, et al. The involvement of annexin A1 in human placental response to maternal Zika virus infection Antiviral Res. 2020 Jul; 179:104809.
役に立ちましたか？
-
-
What type of IL-12p40 was detected in the Human Cytokine Panel A (HCYTA-60K) – IL-12p40 monomer or IL-12p40 total (both monomer and homodimer)?
1 回答-
The capture in the assay detects the IL-12p40 monomer, and the standard used is also the IL-12p40 monomer. However, specific testing has not been conducted to determine if it also detects the homodimer.
役に立ちましたか？
-
-
Can the Milliplex Human Cytokine/Growth Factor Panel A (HCYTA-60K) measure IFNa2a or IFNA2B?
1 回答-
Based on the antibody and standard specification sheets, the kit detects both IFNa2a and IFNa2B. The capture antibody is listed as an IFNa2B antibody and does not mention IFNa2a. However, the standard is recombinant IFNa2a.
役に立ちましたか？
-
-
Are the standards used in Human Cytokine Panel A expressed in E. Coli?
1 回答-
The IFNa2 protein is expressed in yeast, while IL-12p40, IL-12p70, and IL-27 proteins are expressed in HEK293. All the other proteins are expressed in E. Coli.
役に立ちましたか？
-
-
Do you have any information about BPA, specifically bisphenol A from the plastic used in the immunoassay kits?
1 回答-
The plastic materials used in the immunoassay kit are BPA-free according to the manufacturer's websites.
役に立ちましたか？
-
-
Is it better to use EDTA vacutainers than heparinized vacutainers when collecting blood from plasma? Does this apply to both humans and mice?
1 回答-
For our Milliplex assays, EDTA plasma is preferred. Care must be taken when using heparin as an anticoagulant since an excess of heparin will provide falsely high values. Use no more than 10 IU heparin per mL of blood collected.
役に立ちましたか？
-
-
Is there an alternative method if a bath sonicator is not available for sonication of beads as mentioned in the Milliplex protocols? Can vortexing for a longer period serve as an alternative?
1 回答-
The Milliplex assays recommend using a sonicator (water bath, Branson B200 or equivalent). Sonication helps break up any aggregated beads and beads stuck to the vial to reduce aggregation and improve counts. If a bath sonicator is not available, you can vortex for up to 2 minutes.
役に立ちましたか？
-
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）