If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
F6428
遊離グリセロール試薬
used for quantitative enzymatic determination of glycerol
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製品名
遊離グリセロール試薬, used for quantitative enzymatic determination of glycerol
InChI
1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
InChI key
PEDCQBHIVMGVHV-UHFFFAOYSA-N
SMILES string
C(C(CO)O)O
form
solid
usage
40 mL sufficient for 50 reactions
color
white to off-white
functional group
hydroxyl
relevant disease(s)
obesity
storage temp.
2-8°C
Quality Level
関連するカテゴリー
1 of 4
当該品目 | G7793 | FG0100 | 374813 |
|---|---|---|---|
| functional group hydroxyl | functional group - | functional group - | functional group - |
| form solid | form - | form - | form liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| color white to off-white | color - | color - | color - |
| usage 40 mL sufficient for 50 reactions | usage - | usage kit sufficient for 1000 reactions | usage - |
Application
Biochem/physiol Actions
Features and Benefits
- プロセスの簡便化：単独の試薬でワークフローを簡素化し、迅速な5分間の反応時間を提供します。
- ユーザーフレンドリーな設計：複数の試薬の計量または混合の必要性が排除され、使いやすさと効率が確保されます。
General description
血清トリグリセリド測定キット（カタログ番号：TR0100）は、血清または血漿試料中のグリセロール、真のトリグリセリド、または総トリグリセリドを評価するための包括的なソリューションを提供します。酵素加水分解を利用することで、トリグリセリドはグリセロールと遊離脂肪酸に変換され、F6428による共役酵素反応による精密な定量化が可能になります。
市販されている多くのトリグリセリド試薬とは異なり、この方法では、内在性のグリセロールとグリセリドのリパーゼ加水分解に由来するグリセロールが識別されます。この先進的技術を使用することで、グリセロール濃度を非常に優れた正確度および信頼度で明確に分析することができます。
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
資料
Lipid Induced Insulin Resistance
関連コンテンツ
Instructions
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How can I determine the shelf life / expiration / retest date of this product?
1 回答-
役に立ちましたか？
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I would like to measure the concentration of free glycerol in the culture supernatant of adipocytes using this reagent with a plate reader instead of a cuvette. What are recommended sample and reagent volumes for a plate reader? Have you ever tested?
1 回答-
This product has not been tested for use with a well plate format. The product instructions call for a total volume of 830 µL, where 10 µL each of water, glycerol standard, and sample are added to cuvettes containing 800 µL of Free Glycerol Reagent. Most 96-well plates have a working volume of 100-300 µL, so the volume of each component would need to be scaled accordingly. Adding 3 µL each of water, glycerol standard, and sample to wells containing 240 µL of Free Glycerol Reagent would result in the same concentrations with a total volume of 249 µL. Regardless, the functionality of a plate reader in this application cannot be guaranteed and would need to be assessed independently.
役に立ちましたか？
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I have an old version of this product (6428) - just one bottle not a kit. I need to reconstitute this but can't find instructions. Can you let me know what to reconstitute in and how much? Thank you!
1 回答-
There are no specific instructions for 6428. F6428 is one bottle of reagent as well. The user guide of F6428 may serve as a reference. A typical reconstitution procedure of F6428 is: Reconstitute the Free Glycerol Reagent with 40 ml of water. After addition of water, stopper the vials, and immediately mix several times by inversion. DO NOT SHAKE. Protect the reagent from light by storing in amber bottles. Please check page 2 of the user guide here:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/352/968/f6428bul.pdf役に立ちましたか？
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
役に立ちましたか？
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Could you kindly send me a package insert and a manual application for determination on automated clinical systems? Many thanks.
1 回答-
The Free Glycerol Reagent is for R&D use only, not for in vitro diagnostic use, drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
役に立ちましたか？
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