Catalog number F7524 is not tested for tetracycline, so we cannot guarantee that the product is tetracycline free. Product F2442 is regularly tested for tetracycline and the results shown in a lot-specific certificate of analysis. The website page for this product is linked here: https://www.sigmaaldrich.com/catalog/product/sigma/f2442?lang=fr®ion=CH
F7524
ウシ胎児血清
non-USA origin, sterile-filtered, suitable for cell culture
Fetal Bovine Serum
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別名:
FBS, FCS, 血清
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50 ML
お問い合わせ
100 ML
￥19,700
500 ML
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出荷予定日2026年2月05日from市川市大町368
この商品について
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由来生物
bovine fetus
品質水準
無菌性
sterile-filtered
製品種目
FBS Classic
組成
hemoglobin, ≤25 mg/dL
由来
non-USA origin
利用可能性
not available in Canada, USA
テクニック
cell culture | mammalian: suitable
不純物
≤10 EU/mL endotoxin
輸送温度
dry ice
保管温度
−20°C
類似した製品をお探しですか？ 訪問 製品比較ガイド
1 of 4
当該品目 | 12003C | F6765 | F0392 |
|---|---|---|---|
| origin non-USA origin | origin Australia origin | origin USA origin | origin USA origin |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| composition hemoglobin, ≤25 mg/dL | composition Hemoglobin, ≤25 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
アプリケーション
ウシ胎児血清は、ロズウェルパーク記念研究所培地（RPMI-1640）やダルベッコ変法イーグル培地（DMEM）[1]などの細胞培養培地のサプリメントとして、種々の細胞株の増殖をサポートするために使用されています。
生物化学的／生理学的作用
ウシ胎児血清（FBS）は、in vitroおよびex vivoでの細胞、組織および器官の培養を助けるために一般に使用される、細胞培養培地のサプリメントとして作用します。FBSは、細胞培養に幅広い機能を提供するので、依然として定評のある培地サプリメントです。FBSは、栄養、増殖因子および付着因子を送達し、無血清培地（SFM）系では再現困難なメカニズムにより細胞を酸化的損傷とアポトーシスから防御します。FBSは、培養中の細胞に適用される多くの未定義の成長促進因子と生存促進因子を提供します。凝固血液由来であり、真核生物、特に哺乳動物の細胞培養に最も広く使用されている未定義のサプリメントです。 FBSは、1950年代から60年代にかけて開発された旧知の培地に、一般的に使用されます。これらには、最小必須培地（MEM）；ダルベッコ変法イーグル培地（DME、DMEM）；イスコフ改変イーグル培地（IMDM）；M199およびロズウェルパーク記念研究所培地（RPMI-1640）などの多くの一般的な培地が含まれます。
特徴および利点
F7524はBiochrom GmbH製品S0115と同じ製品です。
アナリシスノート
- エンドトキシンおよびヘモグロビン試験済み
- 細菌、ウイルス、およびマイコプラズマの存在についてテスト済み
- 無菌条件下で0.1ミクロンの膜により三重ろ過済み
法的情報
米国およびカナダでは販売していません。在庫と輸入制限につきましてはお問い合わせください。
関連製品
製品番号
詳細
価格
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
F7524-VAR:
F7524-20ML:
F7524-500ML:
F7524-20ML-J:
F7524-100ML:
F7524-BULK:
F7524-50ML:
Carlo E A Jochems et al.
Alternatives to laboratory animals : ATLA, 30(2), 219-227 (2002-04-25)
Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.
Elisabeth F P Peterse et al.
BMC cancer, 16, 475-475 (2016-07-16)
Chondrosarcoma is a malignant cartilage forming bone tumour for which no effective systemic treatment is available. Previous studies illustrate the need for a better understanding of the role of the IGF pathway in chondrosarcoma to determine if it can be
Hans F N Kvitvang et al.
Journal of chromatography. A, 1370, 70-79 (2014-12-03)
Metabolic profiling has become an important tool in biological research, and the chromatographic separation of metabolites coupled with mass spectrometric detection is the most frequently used approach for such studies. The establishment of robust chromatographic methods for comprehensive coverage of
Jiří Voller et al.
Bioorganic chemistry, 90, 103005-103005 (2019-07-05)
Rho-associated serine/threonine kinases (ROCKs) are principal regulators of the actin cytoskeleton that regulate the contractility, shape, motility, and invasion of cells. We explored the relationships between structure and anti-ROCK2 activity in a group of purine derivatives substituted at the C6
Michelle J Nyhan et al.
BMC cancer, 16, 101-101 (2016-02-18)
Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells
資料
細胞培養にウシ胎児（または子ウシ）血清を使用する際の基本と、調達や規制が細胞アッセイ用血清の価格にどのように影響するのかご確認ください。
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
関連コンテンツ
メルクのウシ胎児血清（FBS）のポートフォリオから、お客様のニーズとご予算に合う最適なFBSをお探しください。血清サンプルのご依頼。
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Does the F7524 Fetal Bovine Serum product contain Tetracycline?
1 回答-
役に立ちましたか？
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When aliquioting FBS, is it best to cool them to 4ºC first or directly at -80ºC
1 回答-
The serum can be aliquoted into smaller, single-use portions and refrozen directly.
役に立ちましたか？
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Hello, I have a question regarding FBS (F7524), for my experiment it is essential there is no dopamine or serotonine in my medium. Is it known wether these substances are present in FBS?
1 回答-
This product has not undergone testing to determine the presence of dopamine or serotonin.
役に立ちましたか？
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Could the presence of insoluble precipitates when thawing the serum indicate contamination?
1 回答-
Thawed serum may contain precipitates and flocculants due to various reasons, such as residual fibrinogen converting to fibrin upon thawing. Centrifugation at 400 xg can remove excess fibrin, while filtering serum is not recommended to avoid protein depletion and clogged filters.
Additional points to consider:
•Cloudiness in human serum products is common and indicates the presence of lipids. Clear, lipid-free human serum products are not available.
•Microscopic serum precipitates may resemble yeast contamination but are actually lipid aggregates that can be dissolved by lipase. They do not show growth in culture or gram staining and can be removed through centrifugation or filtration.
•Gel layer or clumps in serum are caused by poor or overheated inactivation or improper mixing of FBS during the Heat inactivation process. Serum with these characteristics should not be used.
•To minimize denaturation of serum lipoproteins and turbidity, it is recommended to prepare single-use aliquots and avoid repeated freeze-thaw cycles.役に立ちましたか？
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Is this product F7524 heat-inactivated? Should it be prior to use?
1 回答-
This product has not been heat inactivated. For most cell culture applications heat inactivation is not necessary. If the application requires inactivation of any heat labile serum components, such as compliment or cytokines, the product may be heat inactivated in the lab. Alternatively, a heat inactivated version of this product is also available. See product F9665.
To heat inactivate this product:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Avoid repeated thaw-freeze cycles.役に立ちましたか？
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Bonjour, Quelle est la composition et la concentration du sérum référencé F7524 en Calcium ? Cordialement
1 回答-
Calcium levels are not determined for this product. The serum is a natural product that will contain calcium at physiological levels.
役に立ちましたか？
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Welches fetale Kälbserum wurde für die Kryokonservierung von Mausembryonen oder Eiestockmäus verwendet?
1 回答-
Unfortunately, a fetal calf serum that has been specifically tested for cryopreservation of mouse embryos or ovarian mice is not currently offered. Please see the link below to review Product ES009-M, Fetal Bovine Serum. This product is tested for expansion and cytotoxicity of mouse embryonic stem cells and may be of interest:
https://www.sigmaaldrich.com/product/mm/es009m役に立ちましたか？
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How can we heat inactivate the fetal bovine serum cat: F7524? For how long?
1 回答-
All heat inactivated serum products are treated at 56 deg. C for 30 minutes. This process may be applied to manually heat inactivate serum on a per bottle basis using a water bath. When immersing bottles do not allow water to come in contact with the cap/closure.
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Is Feta bovine serum F7524-500ML heat inactivated?
1 回答-
This product is not heat-inactivated.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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