The product is essentially a salt-free preparation and should be reconstituted to a minimum concentration of 1 mg/ml with a saline solution containing 150 mM NaCl.
I4506
IgG ヒト血清由来
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
別名:
ヒトIgG
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製品名
IgG ヒト血清由来, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
conjugate
unconjugated
grade
reagent grade
assay
≥95% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
composition
Protein, ≥90%
technique(s)
ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable
storage temp.
2-8°C
Quality Level
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当該品目 | I5131 | I4381 | 56834 |
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form fibers |
| technique(s) ELISA: suitable, immunoelectrophoresis: suitable, dot immunobinding: suitable, western blot: suitable | technique(s) - | technique(s) ELISA: suitable | technique(s) radioimmunoassay: suitable |
| grade reagent grade | grade reagent grade | grade reagent grade | grade - |
| assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (GE) |
Application
Biochem/physiol Actions
IgG抗体サブタイプは、最も豊富な免疫システム血清イムノグロブリンです。B細胞によって分泌され、血中や細胞外液中に存在し、細菌、真菌、およびウイルスによる感染から保護します。母親のIgGは、胎盤を通じて胎児に送られます。これは、免疫防御に重要であり、新生児を感染から守ります。[4]
Disclaimer
弊社カタログまたは製品に付随する他社の文書内に別途記述のある場合を除き、弊社の製品は研究のみでの使用を意図したものであり、無許可での市販、in vitro診断、ex vivoまたはin vivoでの治療、あるいは、ヒトまたは動物に対するすべての種類の消費またはアプリケーションを含む、他のいかなる目的にも使用できません。
General description
精製ヒトIgGは、プールされた正常ヒト血清から、分画およびイオン交換クロマトグラフィーにより分離されます。本製品は、本質的に無塩（ナトリウムが1%未満）の凍結乾燥粉末として供給されます。
Related product
製品番号
詳細
価格
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Nils A Brechmann et al.
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資料
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
プロトコル
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
関連コンテンツ
Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.
Datasheet
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How is this standard reconstituted?
1 回答-
役に立ちましたか？
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What is the expected MW of the product?
1 回答-
The molecular weight of this product is not determined on a lot to lot basis. According to published literature, the molecular weight of Immunoglobulin G (IgG) is approximately 150 - 160 KDa. Historical data available for this product indicates that in SDS-PAGE electrophoresis this material will have multiple bands ranging from 155 kDa - 220 kDa.
役に立ちましたか？
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Necesito saber la secuencia de la proteína o el número para buscarla en uniprot
1 回答-
The protein sequence is not determined for this product. Human IgG has 4 subclasses: IgG1, IgG2, IgG3 and IgG4. These are present in serum at approximately 66%, 23%, 7% and 4%, respectively.
役に立ちましたか？
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What is the isoelectric point of this
1 回答-
This product is not tested on a lot-to-lot basis to determine the isoelectric point. However, historical data indicates an isoelectric point range of 6.1 - 8.5.
役に立ちましたか？
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Is product I4506, because it is derived from human serum, considered to potential biohazard?
1 回答-
As indicated in the corresponding Data Sheet at the link below: "BIOHAZARD: Handle as if capable of transmitting infectious agents". The source material is tested and found negative for antibody to HIV, antibody to HCV, and for HbsAG.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdf役に立ちましたか？
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What is the recommended storage method for item I4506, IgG from human serum, after reconstitution?
1 回答-
After reconstitution, keep aliquots at 2-8°C for short-term use (up to 4 weeks) and store the remaining aliquots at -20°C.
役に立ちましたか？
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What is the significance of absorbance at 280 nm (E280 1% = 14)?
1 回答-
The absorbance at 280 nm (E280 1% = 14) indicates the percent extinction coefficient (ϵ1%).
役に立ちましたか？
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what is the expected molecular weight under reducing conditions
1 回答-
I4506, Human IgG, is a tetrameric protein consisting of 2 light chains and 2 heavy. When run on a reducing gel, The heavy chain will be detectable at ~50kDa, and the light chain at ~25kda.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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Is Product I4506, IgG from human serum, tested for endotoxin?
1 回答-
No. Product No. I4506 is not tested for endotoxin levels.
役に立ちましたか？
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