P1435
酸性ホスファターゼ from sweet potato
ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)
別名:
(オルトリン酸モノエステルホスホヒドロラーゼ[酸性至適型])
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500 UNITS
￥58,500
2500 UNITS
￥256,000
この商品について
CAS番号:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-630-9
MDL number:
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製品名
酸性ホスファターゼ from sweet potato, ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)
SMILES string
O(CC(O)C)CC#C
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
form
ammonium sulfate suspension
specific activity
≥10.0 units/mg protein (modified Warburg-Christian)
secondary activity
≤9.9 units/mg protein ATPase
mol wt
110 kDa
storage temp.
2-8°C
Quality Level
1 of 4
当該品目 | SRE0048 | P1146 | P3752 |
|---|---|---|---|
| specific activity ≥10.0 units/mg protein (modified Warburg-Christian) | specific activity 3.0-10.0 unit/mg solid | specific activity ≥3.0 units/mg solid | specific activity ≥0.5 unit/mg solid |
| form ammonium sulfate suspension | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt 110 kDa | mol wt - | mol wt 69 kDa | mol wt 69 kDa |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 400 | Quality Level 300 | Quality Level 200 |
| secondary activity ≤9.9 units/mg protein ATPase | secondary activity - | secondary activity - | secondary activity - |
Other Notes
1ユニットは、pH 4.8、37°C、1分間で、1.0 μmolのp-ニトロフェニルリン酸を加水分解する酵素量です。
Physical form
1.8 M (NH4)2SO4, 10 mM MgCl2中に分散させた懸濁液(pH 5.3)
Application
Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or α-amylase. [1]
Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. [2] It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase. [3]
Biochem/physiol Actions
Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO4 + H2O --------------- R-OH + HOPO3 2+Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, Apases may be as important as kinases in regulatory processes.
General description
Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points. [4]
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Tatsuya Kusudo et al.
Bioscience, biotechnology, and biochemistry, 67(7), 1609-1611 (2003-08-13)
Purple acid phosphatase (PAP) was purified from sweet potato dry powder, which is used as a food additive. Spectrometric and enzymatic analyses, and analysis of the amino-terminal sequence indicated that the purified purple acid phosphatase was PAP1. High activity in
Mina Solhtalab et al.
Environmental science & technology, 56(22), 16441-16452 (2022-10-26)
Among ubiquitous phosphorus (P) reserves in environmental matrices are ribonucleic acid (RNA) and polyphosphate (polyP), which are, respectively, organic and inorganic P-containing biopolymers. Relevant to P recycling from these biopolymers, much remains unknown about the kinetics and mechanisms of different
D T Wyatt et al.
Clinical chemistry, 35(11), 2173-2178 (1989-11-01)
We analyzed extensively a modified thiochrome method for thiamin analysis. Acid phosphatase (EC 3.1.3.2) from potato was superior to either alpha-amylase or acid phosphatase from wheat germ as a dephosphorylating agent. Timing of cyanogen bromide exposure was important, but the
An unstable manganese(III) complex incorporating ligand donor types proposed for an acid phosphatase from sweet potato: (p-nitrobenzenethiolato)[N,N'-ethylenebis(salicylideneaminato)]manganese(III)
Gohdes, J. and W. Armstrong
Inorganic Chemistry, 27, 1841-1842 (1988)
S M Duff et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(21), 9538-9542 (1991-11-01)
Induction of phosphatase activity is an important component of the plant cell response to phosphate deficiency. Suspension cell cultures of Brassica nigra contain two major inducible acid phosphatase (APase) isozymes; vacuolar phosphoenolpyruvate (PEP) APase and cell wall nonspecific APase. Polyclonal
資料
硫酸アンモニウム懸濁液として提供される酵素の使用方法
Instructions for working with enzymes supplied as ammonium sulfate suspensions
プロトコル
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
関連コンテンツ
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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