HPLC-DAD Analysis of Metformin Hydrochloride on Ascentis® Express C18 HPLC Column
材料
used together
Milli-Q® IQ 7000超純水製造装置
output: type 1 water (18.2 MΩ·cm), the most advanced Milli-Q® ultrapure (Type 1) water purification system that is intelligent, intuitive, and green.CONDITIONS
column
Ascentis® C18, 25 cm × 4.6 mm I.D., 5 μm particle size (581325-U)
mobile phase
[A] 20 mM Potassium hexafluorophosphate in water; [B] Methanol
gradient
95%A to 55%A in 8min, 8-10 min 55%A, 10-16 min 95%A
flow rate
1.0 mL/min
column temp.
25 °C
detector
(DAD, wavelength: 230 nm)
injection
(5 µL)
sample/matrix
0.1 mg/mL in water (PHR1084)
詳細
詳細
Metformin is a biguanide hypoglycemic agent and an oral antidiabetic medication widely used in the treatment of type 2 diabetes. It is effective in glycemic control and decreases the intestinal absorption of glucose, without the problem of serious lactic acidosis.
Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards. They are provided with a comprehensive Certificate of Analysis (COA) included a certified value and expanded uncertainty according to ISO 17034. Additionally, traceability to the current batch of Compendial Reference Standards from USP, EDQM and/or BP is provided, as available.
sigmaaldrich.com/pharmastandards
その他情報
APP_788
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany