05-1427

Anti-ARF1 Antibody, clone 3F1

clone 3F1, from mouse

• Synonym(s): ADP-ribosylation factor 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3F1, monoclonal
• species reactivity: rat, human, mouse
• technique(s): ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NM_001024226.1
• UniProt accession no.: P84077
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ARF1(375) ; mouse ... Arf1(11840)

Description

General description

ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific.

~21 kDa

Immunogen

Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.

Application

Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).

ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer

Biochem/physiol Actions

Recognizes human ARF1

Physical form

Format: Purified

Purified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide

Analysis Note

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.

Other Notes

Replaces: MAB3779

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable