- 05321MG
05-321MG
Anti-Phosphotyrosine Antibody, clone 4G10®
clone 4G10®, Upstate®, from mouse
Recommended Products
biological source
mouse
Quality Level
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
4G10®, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2bκ
shipped in
wet ice
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This Item | 05-321 | 05-777 | 16-104 |
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| antibody form purified from hybridoma cell culture | antibody form purified antibody | antibody form purified immunoglobulin | antibody form - |
| clone 4G10®, monoclonal | clone 4G10®, monoclonal | clone 4G10®, monoclonal | clone 4G10®, monoclonal |
| manufacturer/tradename Upstate® | manufacturer/tradename Upstate® | manufacturer/tradename Upstate® | manufacturer/tradename Upstate® |
| technique(s) immunoprecipitation (IP): suitable, western blot: suitable | technique(s) immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) immunoprecipitation (IP): suitable, western blot: suitable | technique(s) immunocytochemistry: suitable |
| isotype IgG2bκ | isotype IgG2bκ | isotype IgG2bκ | isotype - |
Application
Quality
Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).
Target description
Physical form
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10 (FOX-NY [NS-1 derivative] myeloma x spleen cells).
Analysis Note
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Other Notes
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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