The immunogen used to prepare this product is Na+/K+ ATPase isolated from the membrane fractions of rabbit kidney outer medulla. This antibody recognizes the ?-1 subunit isoform of Na+/K+ ATPase. Consequently, there is a likelihood of generating multiple epitopes within that region, potentially spanning both intra- and extracellular domains. The specific epitope has not been determined nor has the domain. At this time, there is no conjugated version available.
05-369
Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6
clone C464.6, Upstate®, from mouse
Synonym(s):
ATPase, Na+/K+ transporting, alpha 3 polypeptide, Na(+)/K(+) ATPase alpha(III) subunit, Na(+)/K(+) ATPase alpha-3 subunit, Na+/K+ -ATPase alpha 3 subunit, Na+/K+ ATPase 3, Sodium pump subunit alpha-3, dystonia 12, sodium pump 3, sodium-potassium-ATPase,
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
C464.6, monoclonal
species reactivity
rabbit, monkey, sheep, mouse, Xenopus, human, pig, canine, rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ATP1A3(478)
Related Categories
1 of 4
This Item | AB9094-I | 06-172-I | ZMS1029 |
|---|---|---|---|
| clone C464.6, monoclonal | clone polyclonal | clone polyclonal | clone C464.6, recombinant monoclonal |
| antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form purified antibody |
| biological source mouse | biological source rabbit | biological source rabbit | biological source mouse |
| species reactivity rabbit, monkey, sheep, mouse, Xenopus, human, pig, canine, rat | species reactivity mouse, rat, human | species reactivity human | species reactivity mouse, rat, human |
| Gene Information human ... ATP1A3(478) | Gene Information human ... ATP1A2(477) | Gene Information rat ... Atp1A1(24211) | Gene Information rat ... Atp1A1(24211) |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
General description
112 kDa
Na+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase). It is found in the plasma membrane of virtually every human cell and is common to all cellular life. It helps maintain cell potential and regulate cellular volume.
In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.
In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.
Immunogen
Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
Application
Detect Na+/K+ ATPase α-1 (sodium/potassium ATPase subunit alpha 1) using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 validated for use in IC, IH, IP & WB.
Immunoprecipitation:
2 µg of a previous lot immuno-precipitated the α-1 subunit from rat brain microsomal preparations.
Immunohistochemistry:
1-10 µg/mL of a previous lot of this antibody was used in immunohistochemistry on ice-cold methanol-fixed tissue.
1. Do not boil microsomes. Warming to 37ºC for 15 minutes is recommended.
2. Microsomes from some tissues may contain sodium pump subunits which are below the level of detection by this antibody.
3. Microsomes derived from tissues which are more difficult to prepare, such as smooth and skeletal muscle, frequently show non-specific cross-reactivity.
2 µg of a previous lot immuno-precipitated the α-1 subunit from rat brain microsomal preparations.
Immunohistochemistry:
1-10 µg/mL of a previous lot of this antibody was used in immunohistochemistry on ice-cold methanol-fixed tissue.
1. Do not boil microsomes. Warming to 37ºC for 15 minutes is recommended.
2. Microsomes from some tissues may contain sodium pump subunits which are below the level of detection by this antibody.
3. Microsomes derived from tissues which are more difficult to prepare, such as smooth and skeletal muscle, frequently show non-specific cross-reactivity.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Biochem/physiol Actions
This antibody recognizes the α-1 subunit isoform of Na+/K+ ATPase.
Physical form
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG1κ in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at
-20ºC.
Produced from a culture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes. Clone C464.6 also called 6H.
-20ºC.
Produced from a culture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes. Clone C464.6 also called 6H.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Alpha 1 mRNA is present in all cell types examined.
Alpha 1 mRNA is present in all cell types examined.
Routinely evaluated in western blot on rat brain microsomal preparations.
Western Blot Analysis:
0.05-0.1 µg/mL of this lot detected the α-1 subunit in 20 µg of rat brain microsomal preparation (Catalog # 12-144). To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.
Western Blot Analysis:
0.05-0.1 µg/mL of this lot detected the α-1 subunit in 20 µg of rat brain microsomal preparation (Catalog # 12-144). To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture.
Pietrini, G, et al.
Proceedings of the National Academy of Sciences of the USA, 89, 8414-8418 (1992)
Renal phenotype of UT-A urea transporter knockout mice.
Fenton, RA; Flynn, A; Shodeinde, A; Smith, CP; Schnermann, J; Knepper, MA
Journal of the American Society of Nephrology null
Vasopressin regulation of the renal UT-A3 urea transporter.
Stewart, GS; Thistlethwaite, A; Lees, H; Cooper, GJ; Smith, C
American Journal of Physiology: Renal Physiology null
Elevated BSC-1 and ROMK expression in Dahl salt-sensitive rat kidneys.
Hoagland, KM; Flasch, AK; Dahly-Vernon, AJ; dos Santos, EA; Knepper, MA; Roman, RJ
Hypertension null
Reorganization of lipid rafts during capacitation of human sperm.
Cross, NL
Biology of reproduction null
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Hello, Is this Ab recognize intracellular or extracellular area of this protein? Do you have a conjugated version of it?
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