05-535

Anti-IKKβ Antibody, clone 10AG2

Name: Anti-IKKβ Antibody, clone 10AG2
Brand: MM
Price: 550 USD

clone 10AG2, Upstate^®, from mouse

Synonym(s):

I-kappa-B kinase 2, I-kappa-B-kinase beta, Nuclear factor NF-kappa-B inhibitor kinase beta, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta, inhibitor of nuclear factor kappa B kinase beta subunit

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100 μG

$550.00

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Key Documents

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Product Name

Anti-IKKβ Antibody, clone 10AG2, clone 10AG2, Upstate^®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10AG2, monoclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Upstate^®

technique(s)

western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001556

UniProt accession no.

O14920

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

mouse ... Ikbkb(16150)

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This Item	06-1340	MABF222	ABS1515
Sigma-Aldrich 05-535 Anti-IKKβ Antibody, clone 10AG2	Sigma-Aldrich 06-1340 Anti-phospho-IKK-epsilon (Ser172) Antibody	Sigma-Aldrich MABF222 Anti-IKKa (p45) Antibody, clone 881H3	Sigma-Aldrich ABS1515 Anti-phospho NFkB p52, (Ser222) Antibody
clone 10AG2, monoclonal	clone polyclonal	clone 881H3, monoclonal	clone polyclonal
antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody
species reactivity rat, human, mouse	species reactivity human, mouse	species reactivity human	species reactivity human
biological source mouse	biological source rabbit	biological source mouse	biological source rabbit
technique(s) western blot: suitable	technique(s) western blot: suitable	technique(s) immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable	technique(s) western blot: suitable
shipped in dry ice	shipped in wet ice	shipped in wet ice	shipped in wet ice

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Routinely evaluated by western blot on human Jurkat RIPA cell lysate, human A431, HeLa nuclear extract, mouse 3T3/A31 or rat PC-12 RIPA cell lysates.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected IKK/IKK2 in human HeLa cell lysate. A previous lot was tested in human Jurkat RIPA cell lysate, A431, extract, mouse 3T3/A31 and rat PC-12 RIPA cell lysates.

Application

Anti-IKKβ Antibody, clone 10AG2 detects level of IKKβ & has been published & validated for use in WB.

Biochem/physiol Actions

Other species not tested.

This antibody recognizes IKKβ/IKK2, Mr 87 kDa.

General description

87 kDa

IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1.

Immunogen

His-tagged, full length human IKKβ/IKK2. Clone: 10AG2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing PBS and 0.05% sodium azide. Frozen solution.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Canonical NF-κB signaling is uniquely required for the long-term persistence of functional mature B cells.

Emmanuel Derudder et al.

Proceedings of the National Academy of Sciences of the United States of America, 113(18), 5065-5070 (2016-04-22)

Although canonical NF-κB signaling is crucial to generate a normal mature B-cell compartment, its role in the persistence of resting mature B cells is controversial. To resolve this conflict, we ablated NF-κB essential modulator (NEMO) and IκB kinase 2 (IKK2)

Identification of TBK1 complexes required for the phosphorylation of IRF3 and the production of interferon β.

Siddharth Bakshi et al.

The Biochemical journal, 474(7), 1163-1174 (2017-02-06)

The double-stranded RNA mimetic poly(I:C) and lipopolysaccharide (LPS) activate Toll-like receptors 3 (TLR3) and TLR4, respectively, triggering the activation of TANK (TRAF family member-associated NF-κB activator)-binding kinase 1 (TBK1) complexes, the phosphorylation of interferon regulatory factor 3 (IRF3) and transcription

Crystal structure of inhibitor of ?B kinase ?.

Xu, G; Lo, YC; Li, Q; Napolitano, G; Wu, X; Jiang, X; Dreano, M; Karin, M; Wu, H

Nature null

Validation of IKK beta as therapeutic target in airway inflammatory disease by adenoviral-mediated delivery of dominant-negative IKK beta to pulmonary epithelial cells.

Matthew C Catley, Joanna E Chivers, Neil S Holden, Peter J Barnes, Robert Newton

British Journal of Pharmacology null

IKK is a therapeutic target in KRAS-Induced lung cancer with disrupted p53 activity.

Daniela S Bassères et al.

Genes & cancer, 5(1-2), 41-55 (2014-06-24)

Activating mutations in KRAS are prevalent in cancer, but therapies targeted to oncogenic RAS have been ineffective to date. These results argue that targeting downstream effectors of RAS will be an alternative route for blocking RAS-driven oncogenic pathways. We and

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