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MilliporeSigma

05-773

Anti-phospho-MYPT1 (Thr850) Antibody, clone SA19, rabbit monoclonal

clone SA19, Upstate®, from rabbit

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100 μL

$405.00

$405.00


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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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Product Name

Anti-phospho-MYPT1 (Thr850) Antibody, clone SA19, rabbit monoclonal, clone SA19, Upstate®, from rabbit

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SA19, monoclonal

species reactivity

human, chicken

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pThr850)

Quality Level

Gene Information

human ... PPP1R12A(4659)

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This Item
36-00304-773ABS45
species reactivity

human, chicken

species reactivity

chicken, rat, human, mouse

species reactivity

rat, chicken, mouse, human

species reactivity

human

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

culture supernatant

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

clone

SA19, monoclonal

clone

polyclonal

clone

SA19, monoclonal

clone

polyclonal

Gene Information

human ... PPP1R12A(4659)

Gene Information

human ... PPP1R12A(4659)

Gene Information

human ... PPP1R12A(4659)

Gene Information

human ... PPP1R12A(4659)

UniProt accession no.

O14974

UniProt accession no.

O14974

UniProt accession no.

O14974

UniProt accession no.

O14974

Analysis Note

Routinely evaluated by immunoblot on RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).

Application

Anti-phospho-MYPT1 (Thr850) Antibody, clone SA19 is an antibody against phospho-MYPT1 (Thr850) for use in WB.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases

Biochem/physiol Actions

MYPT1 phosphorylated on Thr850
Predicted to cross react with mouse and rat

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

doublet at 130kDa

Immunogen

KLH-conjugated, synthetic peptide containing the sequence ...RS[pT]GV..., in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.

Physical form

Format: Purified
Protein A purified

Preparation Note

2 years at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Role of protein phosphatase type 1 in contractile functions: myosin phosphatase.
Hartshorne, David J, et al.
The Journal of Biological Chemistry, 279, 37211-37214 (2004)
Sustained therapeutic hypercapnia attenuates pulmonary arterial Rho-kinase activity and ameliorates chronic hypoxic pulmonary hypertension in juvenile rats.
Peng, G; Ivanovska, J; Kantores, C; Van Vliet, T; Engelberts, D; Kavanagh, BP; Enomoto et al.
American Journal of Physiology. Heart and Circulatory Physiology null
Giuseppe Penna et al.
The Prostate, 69(5), 480-493 (2008-12-25)
Benign prostatic hyperplasia (BPH) is characterized by an important inflammatory component. Stimulation of human prostate stromal cells from BPH tissues with proinflammatory cytokines leads to secretion of IL-8, a chemokine involved in BPH pathogenesis. The vitamin D receptor (VDR) agonist
Hypertension and RhoA/Rho-kinase signaling in the vasculature: highlights from the recent literature.
Lee, Dexter L, et al.
Hypertension, 44, 796-799 (2004)
Myosin phosphatase: structure, regulation and function.
Ito, Masaaki, et al.
Molecular and Cellular Biochemistry, 259, 197-209 (2004)

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