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06-332

Sigma-Aldrich

Anti-GST Tag Antibody

Upstate®, from rabbit

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eCl@ss:
32160702
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

clone

polyclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

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This Item
05-31116-209SAB1306083
Anti-GST Tag Antibody Upstate®, from rabbit

Sigma-Aldrich

06-332

Anti-GST Tag Antibody

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

clone

polyclonal

clone

DG122-2A7, monoclonal

clone

DG122-2A7, monoclonal

clone

polyclonal

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

technique(s)

western blot: suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: 1:1000

isotype

IgG

isotype

IgG

isotype

IgG

isotype

-

Immunogen

GST (Glutathione-S-transferase) derived from the pGEX expression vector and linked to KLH

Application

Anti-GST Tag Antibody detects level of GST Tag & has been published & validated for use in WB.

Quality

routinely evaluated by immunoblot on MAP Kinase 2/Erk2, active (Catalog # 14-173). 0.5-2mg/ml of a previous lot detected 500ng of GST (Catalog # 12-350)

Target description

28-30 kDa

Physical form

Format: Purified

Analysis Note

Control
Cells expressing the GST-tag

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Laura Megrelis et al.
Frontiers in immunology, 9, 2001-2001 (2018-09-27)
We previously identified Fam65b as an atypical inhibitor of the small G protein RhoA. Using a conditional model of a Fam65b-deficient mouse, we first show that Fam65b restricts spontaneous RhoA activation in resting T lymphocytes and regulates intranodal T cell
Functional domains of a geminivirus replication protein
Orozco, B. M., et al
The Journal of Biological Chemistry, 272, 9840-9846 (1997)
The telomeric protein TRF2 binds the ATM kinase and can inhibit the ATM-dependent DNA damage response.
Karlseder, J; Hoke, K; Mirzoeva, OK; Bakkenist, C; Kastan, MB; Petrini, JH; de Lange, T
PLoS Biology null
Andrea Brenna et al.
eLife, 8 (2019-11-07)
Circadian oscillations emerge from transcriptional and post-translational feedback loops. An important step in generating rhythmicity is the translocation of clock components into the nucleus, which is regulated in many cases by kinases. In mammals, the kinase promoting the nuclear import
Lotus japonicus E3 ligase SEVEN IN ABSENTIA4 destabilizes the symbiosis receptor-like kinase SYMRK and negatively regulates rhizobial infection.
Den Herder, G; Yoshida, S; Antolin-Llovera, M; Ried, MK; Parniske, M
Plant Cell null

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