07-353

Anti-acetyl-Histone H3 (Lys14) Antibody

serum, Upstate^®

• Synonym(s): H3K14Ac, Histone H3 (acetyl K14), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: Saccharomyces cerevisiae, yeast, human
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable (ChIP-seq); dot blot: suitable; multiplexing: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_003493
• UniProt accession no.: Q16695
• shipped in: dry ice
• Gene Information: human ... H3C1(8350)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.

Specificity

Broad species-cross reactivity is expected.

Recognizes acetyl-histone H3 (Lys14), Mr ~17 kDa. An additional unknown protein was detected at Mr ~33 kDa.

Immunogen

Epitope: a.a. 9-18

Ovalbumin-conjugated, synthetic peptide (KSTGGAcKAPRK-C) corresponding to amino acids 9-18 of yeast histone H3 acetylated on lysine 14, with a C-terminal cysteine added for conjugation purposes.

Application

Anti-acetyl-Histone H3 (Lys14) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as H3K14Ac, Histone H3 (acetyl K14) & has been validated in ChIP, WB, ChIP-seq, DB, Mplex.

Chromatin Immunoprecipitation (ChIP):
An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from yeast strains containing a Lysine substitution to Alanine at residue 14.

Beadlyte Histone-Peptide Specificity Assay:
1:1000-1:5000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. No cross-reactivity with peptides containing acetyl-lysine 9 or acetyl-lysine 27 was detected.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Quality

In acid extracts from sodium butyrate treated HeLa cells.

Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected acetyl-Histone H3 (Lys14) in acid extracts from sodium butyrate treated HeLa cells (Catalog # 17-305).

Target description

17 kDa

Linkage

Replaces: 04-1044

Physical form

Antiserum containing 0.05% sodium azide and 30% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
C6 cell lysate, NIH 3T3 cell lysate, human breast carcinoma and human lung carcinoma.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable