The efficiency available for the separation (and by this resolution) is always dependent on column length. Reducing the column length to half is reducing the efficiency of the column to half. The principle is the same with RP and HILIC - there is no difference.
1.50440
SeQuant® ZIC®-HILIC 100Å (3.5 µm) HPLC Columns
L × I.D. 50 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel
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About This Item
UNSPSC Code:
41115709
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Product Name
SeQuant® ZIC-HILIC™ 3.5µm,100Å 50 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Level
agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
parameter
550 bar pressure
70 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
5 cm × 2.1 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
3.5 μm
pore size
100 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
1 of 4
This Item | 1.50445 | 1.50446 | 1.50447 |
|---|---|---|---|
| matrix active group ZIC-HILIC bonding phase | matrix active group ZIC-HILIC bonding phase | matrix active group ZIC-HILIC bonding phase | matrix active group ZIC-HILIC bonding phase |
| particle size 3.5 μm | particle size 3.5 μm | particle size 3.5 μm | particle size 3.5 μm |
| separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) |
| matrix fully porous particle | matrix fully porous particle | matrix fully porous particle | matrix fully porous particle |
| pore size 100 Å pore size | pore size 200 Å pore size | pore size 200 Å pore size | pore size 200 Å pore size |
| product line SeQuant® | product line SeQuant® | product line SeQuant® | product line SeQuant® |
General description
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.The column dimension of 50 mm length x 2.1 mm ID is the optimal choice for the fast and high resolution separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide
Analysis Note
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.40
Symmetry (Cytosine): 0.95 - 1.40
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Do they have concerns about potential performance issues when opting for a smaller dimension column,considering that this is a HILIC column? Especially for the analysis of sulfites using the 150 x 2.1, 5µm (100 A) column for the conversion of free sulfites to HMS (hydroxymethylsulfonate) and subsequent analysis by LC-MS/MS. Is there a risk of reduced performance when using a column size of 50 x 2.1 mm, 3.5 µ (100 A) instead of the larger format for SeQuant ZIC-HILIC columns?
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