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23-024-M

p27 Protein complex, activated, 10 µg

Activated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.

Synonym(s):

p27 kip1, Cyclin-dependent kinase inhibitor 1B, Kip1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405

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Product Name

p27 Protein complex, activated, 10 µg, Activated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

GenBank accession no.

BC001971 (p27)

NCBI accession no.

NM_001238 (Cyclin E1)
NM_001798

Quality Level

100

Gene Information

human ... CDK2(1017), CDKN1B(1027)

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This Item
427M-923-023-M23-030-M
p27 Protein complex, activated, 10 µg Activated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.

Sigma-Aldrich

23-024-M

p27 Protein complex, activated, 10 µg

p27Kip1 (SX53G8) Mouse Monoclonal Antibody

Sigma-Aldrich

427M-9

p27Kip1 (SX53G8) Mouse Monoclonal Antibody

SCF-Skp2 Protein Complex, Active, 10 µg Active complex of five (5) full length, recombinant human enzymes: Skp1, Skp2, Cul1, Rbx1 & Cks1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

Sigma-Aldrich

23-023-M

SCF-Skp2 Protein Complex, Active, 10 µg

SCF-Fbw7 Protein Complex, Active, 10 µg Active complex of four (4) full length, recombinant human enzymes: Fbw7, Skp1, Cul1 & Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

Sigma-Aldrich

23-030-M

SCF-Fbw7 Protein Complex, Active, 10 µg

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

-

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

biological source

human

biological source

mouse

biological source

human

biological source

human

technique(s)

activity assay: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

Gene Information

human ... CDK2(1017), CDKN1B(1027)

Gene Information

rat ... Cdkn1B(83571)

Gene Information

human ... Cks1(1163), Cul1(8454), Rbx1(9978), Skp1(6500), Skp2(6502)

Gene Information

human ... Cul1(8454), Fbw7(55294), Rbx1(9978), Skp1(6500)

NCBI accession no.

NM_001238 (Cyclin E1), NM_001798

NCBI accession no.

-

NCBI accession no.

NM_001826, NM_003592, NM_005983, NM_014248, NM_170679

NCBI accession no.

NM_003592, NM_014248, NM_033632, NM_170679

Quality Level

100

Quality Level

100, 500

Quality Level

100

Quality Level

100

General description

p27 is an important regulator of cell cycle progression, involved in G1 arrest. It is a potent inhibitor of cyclin E1- and cyclin A-CDK2 complexes and a positive regulator of cyclin D-dependent kinases such as CDK4. The degradation of this protein, which is triggered by its CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Decreased levels of p27 are found in various epithelial tumours originating from lung, breast, colon, ovary, oesophagus, thyroid and prostate. Defects in the gene encoding p27 (CDKN1B) are the cause of multiple endocrine neoplasia type 4 (MEN4) an inherited cancer of the thyroid.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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