For Flow Cytometry, you are to prepare an antibody working solution by diluting the primary antibody 1:5 with PBS. For a standard Flow Cytometry instrument, you will use 10uL of the working solution with 1x10^6 cells in a final reaction volume of 100 microliters.
FCMAB317PE
Milli-Mark® Anti-NeuN-PE Antibody, clone A60
clone A60, Milli-Mark®, from mouse
Synonym(s):
Neuron-Specific Nuclear Protein, Neuna60, A60
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
PE
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
A60, monoclonal
species reactivity
human
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RBFOX3(146713)
Related Categories
1 of 4
This Item | MAB377B | MAB377 | MAB377X |
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| conjugate PE | conjugate biotin conjugate | conjugate - | conjugate ALEXA FLUOR™ 488 |
| species reactivity human | species reactivity rat, mouse | species reactivity avian, pig, chicken, human, rat, salamander, ferret, mouse | species reactivity human, rat, mouse |
| clone A60, monoclonal | clone A60, monoclonal | clone A60, monoclonal | clone A60, monoclonal |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| Gene Information human ... RBFOX3(146713) | Gene Information human ... RBFOX3(146713) | Gene Information human ... RBFOX3(146713) | Gene Information human ... RBFOX3(146713) |
General description
NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5.
Immunogen
Purified cell nuclei from mouse brain.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
This Milli-Mark Anti-NeuN-PE Antibody, clone A60 is validated for use in FC for the detection of NeuN.
Biochem/physiol Actions
Antibody recognizes Neuronal Nuclei.
Physical form
Protein A purified
Purified mouse monoclonal IgG1 conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA
Preparation Note
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
U251 cells
U251 cells
Evaluated by flow cytometry using U251 cells.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Optimal dilution of antibody FCMAB317PE for Flow in 1x10^6 cells? 1:100?
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