MAB1083

Anti-GFP Antibody, clone 3F8.2

clone 3F8.2, from mouse

• Synonym(s): Green Fluorescent protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3F8.2, monoclonal
• species reactivity: vertebrates
• technique(s): western blot: suitable
• isotype: IgG1κ
• UniProt accession no.: P42212
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

28 kDa

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Immunogen

Epitope: Full Length

Full length GFP protein.

Application

Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Epitope Tags

Western Blot Analysis:
0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).

Biochem/physiol Actions

Reacts with most common vertebrate proteins containing the GFP tag.

The antibody reacts with the 28 kDa GFP protein.

Physical form

Format: Purified

Protein G Purified

Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Western Blot: GFP recombinant protein

Routinely evaluated by Western Blot on GFP recombinant protein.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable