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MAB2160

Sigma-Aldrich

Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3

ascites fluid, clone 1C3, Chemicon®

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Synonym(s):
FMRP
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1C3, monoclonal

species reactivity

mouse, human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1κ

suitability

not suitable for flow cytometry
not suitable for immunoprecipitation

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

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This Item
F4055F1554WH0002332M1
antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

clone

1C3, monoclonal

clone

polyclonal

clone

A42, monoclonal

clone

2D4, monoclonal

species reactivity

mouse, human, rat

species reactivity

human, mouse, rat

species reactivity

human

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cells lysate, indirect immunofluorescence: 2-5 μg/mL using methanol-acetone fixed heat-shocked NIH3T3 cells, western blot: 1-2 μg/mL using HEK-293T cell lysate, western blot: 2-4 μg/mL using RAT1 cell lysate

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 2-4 μg/mL using total cell extract of HEK 293T cells

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

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General description

Fragile X Mental Retardation Protein (FMRP) is a RNA-binding protein that is associated to polysomes and may be involved in the transport of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.

Specificity

Human and mouse FMRP. The epitope is localized in the N-terminal half of FMRP. Cross reaction with FXR protein may be detected in cases of high expression of the latter proteins.

Immunogen

Fusion protein with a full length FMRP (human).

Application

Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3 detects level of Fragile X Mental Retardation Protein & has been published & validated for use in ELISA, IC, IH, IH(P) & WB.
Immunohistochemistry:
Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.

ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.

Detection of FMRP on Blood Smears:
A 1:500-1:5,000 dilution of a previous lot was used.

Immunocytochemistry:
A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

RNA Binding Protein (RBP)

Quality

Evaluated by Western Blot on Mouse E17 spinal cord lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected Fragile X Mental Retardation Protein on 10 μg of Mouse E17 spinal cord lysates.

Target description

~71 kDa

Physical form

Ascites mouse monoclonal IgG1k fluid containing no preservatives
Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity.
Bartley, CM; O'Keefe, RA; Bordey, A
Testing null
Gabi Schutzius et al.
Journal of neurodevelopmental disorders, 5(1), 8-8 (2013-04-04)
Hypermethylation of the fragile X mental retardation 1 gene FMR1 results in decreased expression of FMR1 protein FMRP, which is the underlying cause of Fragile X syndrome - an incurable neurological disorder characterized by mental retardation, anxiety, epileptic episodes and
The RNA-binding protein fragile X-related 1 regulates somite formation in Xenopus laevis.
Huot, ME; Bisson, N; Davidovic, L; Mazroui, R; Labelle, Y; Moss, T; Khandjian, EW
Molecular Biology of the Cell null
Julie B Zang et al.
PLoS genetics, 5(12), e1000758-e1000758 (2009-12-17)
The mental retardation, autistic features, and behavioral abnormalities characteristic of the Fragile X mental retardation syndrome result from the loss of function of the RNA-binding protein FMRP. The disease is usually caused by a triplet repeat expansion in the 5'UTR
Brady P Culver et al.
Journal of Huntington's disease, 5(1), 39-51 (2016-02-19)
The Huntington's disease (HD) protein huntingtin (Htt) plays a role in multiple cellular pathways. Deregulation of one or more of these pathways by the mutant Htt protein has been suggested to contribute to the disease pathogenesis. Our recent discovery-based proteomics

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