MAB2259Z

Anti-Integrin β1 Antibody, activated, clone B44, azide free

Name: Anti-Integrin β1 Antibody, activated, clone B44, azide free
Brand: MM
Price: 534 USD

clone B44, Chemicon^®, from mouse

Synonym(s):

Ligand-inducedBindingSite, LIBS, CLIBS, MAB2259

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100 μG

$534.00

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

Key Documents

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biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B44, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon^®

technique(s)

cell culture | mammalian: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002292.3

UniProt accession no.

P55268

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ITGB1(3688)

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This Item	MAB2251	MAB1951Z	MAB2250Z
Sigma-Aldrich MAB2259Z Anti-Integrin β1 Antibody, activated, clone B44, azide free	Sigma-Aldrich MAB2251 Anti-Integrin β1 Antibody, clone B3B11	Sigma-Aldrich MAB1951Z Anti-Integrin β1 Antibody, clone P4G11, azide free	Sigma-Aldrich MAB2250Z Anti-Integrin β1 Antibody, clone 21C8, azide free
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
species reactivity human	species reactivity human	species reactivity monkey, human, rabbit	species reactivity human
clone B44, monoclonal	clone B3B11, monoclonal	clone P4G11, monoclonal	clone 21C8, monoclonal
Gene Information human ... ITGB1(3688)	Gene Information human ... ITGB1(3688)	Gene Information human ... ITGB1(3688)	Gene Information human ... ITGB1(3688)
biological source mouse	biological source mouse	biological source mouse	biological source mouse
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100

General description

88 kDa

Application

Immunoprecipitation of detergent-solubilized (1 integrins: 2-4 μg/IP

Western blot: 10-15 μg/mL

Flow Cytometry: 10-15 μg/mL

Immunohistochemistry

Stimulation of (1-mediated adhesion: 10-20 μg/mL.

Optimal working dilutions must be determined by end user.

This Anti-Integrin β1 Antibody, activated, clone B44, azide free is validated for use in FC, IH, IP, FUNC, CULT, WB for the detection of Integrin β1.

Biochem/physiol Actions

Detects a cation- and ligand-induced binding site (LIBS) of integrin beta 1, which has been used in some systems as a reporter of integrin functionality.

Physical form

Format: Purified

Purified immunoglobulin. Liquid PBS, containing no preservatives.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fibronectin in cell adhesion and migration via N-glycosylation.

Cheng-Te Hsiao et al.

Oncotarget, 8(41), 70653-70668 (2017-10-21)

Directed cell migration is an important step in effective wound healing and requires the dynamic control of the formation of cell-extracellular matrix interactions. Plasma fibronectin is an extracellular matrix glycoprotein present in blood plasma that plays crucial roles in modulating

Dopamine interacts directly with its D3 and D2 receptors on normal human T cells, and activates beta1 integrin function.

M Levite et al.

European journal of immunology, 31(12), 3504-3512 (2001-12-18)

Dopamine by itself has not up to now been reported to activate T cell function. We show here that dopamine interacts directly with dopaminergic receptors on normal human T cells and triggers beta1 integrin-mediated T cell adhesion to a major

Regulation of proximal tubular cell differentiation and proliferation in primary culture by matrix stiffness and ECM components.

Wan-Chun Chen et al.

American journal of physiology. Renal physiology, 307(6), F695-F707 (2014-07-25)

To explore whether matrix stiffness affects cell differentiation, proliferation, and transforming growth factor (TGF)-β1-induced epithelial-mesenchymal transition (EMT) in primary cultures of mouse proximal tubular epithelial cells (mPTECs), we used a soft matrix made from monomeric collagen type I-coated polyacrylamide gel

Estrogen's Effects on Excitatory Synaptic Transmission Entail Integrin and TrkB Transactivation and Depend Upon β1-integrin function.

Weisheng Wang et al.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 41(11), 2723-2732 (2016-06-09)

Estradiol (E2) perfusion rapidly increases the strength of fast excitatory transmission and facilitates long-term potentiation in the hippocampus, two effects likely related to its memory-enhancing properties. Past studies showed that E2's facilitation of transmission involves activation of RhoA signaling leading

Osteopontin binding to the alpha 4 integrin requires highest affinity integrin conformation, but is independent of post-translational modifications of osteopontin.

Tommy Hui et al.

Matrix biology : journal of the International Society for Matrix Biology, 41, 19-25 (2014-12-03)

Osteopontin (OPN) is a ligand for the α4ß1 integrin, but the physiological importance of this binding is not well understood. Here, we have assessed the effect of post-translational modifications on OPN binding to the α4 integrin on cultured human leukocyte

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