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MAB8140

Sigma-Aldrich

Anti-Cytomegalovirus Antibody, early, clone 5A8.2

clone 5A8.2, Chemicon®, from mouse

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Synonym(s):
CMV
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

ascites fluid

clone

5A8.2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

shipped in

wet ice

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This Item
MAB8126MAB8121MAB1978
clone

5A8.2, monoclonal

clone

2D4.2, monoclonal

clone

1G5.2, monoclonal, 2D4.2, monoclonal, 8B1.2, monoclonal

clone

LM142, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

isotype

IgG1

isotype

IgG2a

isotype

IgG2a

isotype

IgG1

Specificity

Reacts with Immediate Early 2 protein (IE2) and its fragments. Recognizes a can epitope near the carboxy-end that includes an epitope found in IE2 86, IE2 60 and IE2 40. In western blot recognizes proteins at apparent molecular weights of 86, 68-72, 55 and 38 kD. Can detect CMV infection 1 hour post-infection exhibiting nuclear and/or intranuclear inclusion staining with reaches peak intensity at 3 hours.


With CMV the antigens expressed at different times are listed as:

Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD

Immunogen

Affinity purified antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Epitope: early

Application

Anti-Cytomegalovirus Antibody, early, clone 5A8.2 detects level of Cytomegalovirus & has been published & validated for use in IF, WB, IH & IP.
Immunochemistry.

IFA at 1:3,000-1:6,000 on acetone fixed cells.

Does not work with paraffin embedded tissue sections.

Dilute with buffer pH 7.4-7.6 to desired working volume.

For extensive dilution, protein containing or other stabilizing medium should be used.

Final working dilutions must be determined by end user.

Physical form

Format: Purified
Purified ascites. Supplied in PBS buffer, pH 7.4. Contains 0.01% sodium azide and carrier protein. Has been sterile filtered.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Christopher B Ball et al.
mBio, 13(3), e0033722-e0033722 (2022-05-18)
Human cytomegalovirus (HCMV) immediate-early 2 (IE2) protein is a multifunctional transcription factor that is essential for lytic HCMV infection. IE2 functions as an activator of viral early genes, negatively regulates its own promoter, and is required for viral replication. The
Yi-Chieh Perng et al.
Journal of virology, 85(10), 4841-4852 (2011-03-04)
In this study, we adopted a conditional protein genetic approach to characterize the role of the human cytomegalovirus (HCMV) gene UL79 during virus infection. We constructed ADddUL79, a recombinant HCMV in which the annotated UL79 open reading frame (ORF) was
The IE2 60-kilodalton and 40-kilodalton proteins are dispensable for human cytomegalovirus replication but are required for efficient delayed early and late gene expression and production of infectious virus.
White, Elizabeth A, et al.
Journal of virology, 81, 2573-2583 (2007)
Development of cell lines that provide tightly controlled temporal translation of the human cytomegalovirus IE2 proteins for complementation and functional analyses of growth-impaired and nonviable IE2 mutant viruses.
Sanders, RL; Clark, CL; Morello, CS; Spector, DH
Journal of virology null
Internal deletions of IE2 86 and loss of the late IE2 60 and IE2 40 proteins encoded by human cytomegalovirus affect the levels of UL84 protein but not the amount of UL84 mRNA or the loading and distribution of the mRNA on polysomes.
Sanders, Rebecca L, et al.
Journal of virology, 82, 11383-11397 (2008)

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