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Anti-N-Myc Antibody, clone NCM II 100

clone NCM II 100, from mouse

N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, bHLHe37

biological source


Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies


NCM II 100, monoclonal

species reactivity



immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... MYCN(4613)

General description

The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.


Recombinant protein corresponding to human N-Myc.


Immunofluorescence Analysis: A representative lot from an independent laboratory detected N-Myc in IMR5 cells (Ikegaki, N., et al. (1986). 83(16):5929-5933.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated N-Myc in IP (Brondyk, W. H., et al. (1991). 6(7):1269-1276.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-N-Myc Antibody, clone NCM II 100 (mouse monoclonal antibody) validated in WB, IP, ICC to detect N-Myc also known as N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, bHLHe37.


Evaluated by Western Blot in IMR-32 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected N-Myc in 10 µg of IMR-32 cell lysate.

Target description

~55 kDa observed. Uniprot describes a molecular weight at ~50 kDa Uniprot states that this protein may be phosphorylated.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

IMR-32 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


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Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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