Documents

FSUSGMMRO

Roche

FastStart Universal SYBR Green Master (Rox)

Quality Level

manufacturer/tradename

Roche

packaging

pkg of 200 x 50 μL reactions (04913850001)
pkg of 2000 x 50 μL reactions (04913914001)

application(s)

qPCR: suitable

General description

FastStart Universal SYBR® Green Master (Rox) is a ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX.

SYBR® Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the SYBR® Green I dye, which is included in the reaction mix, binds to the amplified PCR products. The amplicon can be detected by its fluorescence.

Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature (+15 to +25°C). Therefore, there is no elongation during the period when primers can non-specifically bind. FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).

Application

FastStart Universal SYBR® Green Master (Rox) can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich.
Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.
FastStart Universal SYBR® Green Master (Rox) has been used in qRT-PCR and qPCR

Features and Benefits

  • Improve PCR sensitivity and specificity.
Minimize the formation of nonspecific amplification products.

  • Avoid over-estimation of qPCR results.
Eliminate nonspecific amplification products and primer-dimers that increase the amount of bound quantified SYBR Green I.
  • Amplify and detect a broad range of DNA or cDNA targets.
Amplify fragments up to 500 bp long, including those that are GC- or AT-rich.

  • Save time and effort in qPCR preparation.
Eliminate the need to mix components, titrate MgCl2, or perform other time-consuming optimization steps.

  • Prevent false positives resulting from carryover contamination.
Use this dUTP-containing mix with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions.

Components

FastStart Universal SYBR Green Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), SYBR Green I, and a reference dye.

Quality

Function test: Each lot is tested for performance in qPCR using three templates: a GC-rich template, an AT-rich template, and a long template (approximately 440 bp).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

FastStart is a trademark of Roche
SYBR is a registered trademark of Life Technologies

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Lysine-specific demethylase 2B (KDM2B)-let-7-enhancer of zester homolog 2 (EZH2) pathway regulates cell cycle progression and senescence in primary cells.
Tzatsos A, et al.
The Journal of Biological Chemistry, 286(38), 33061-33069 (2011)
The Kruppel-like factor 15 as a molecular link between myogenic factors and a chromosome 4q transcriptional enhancer implicated in facioscapulohumeral dystrophy.
Dmitriev P, et al.
The Journal of Biological Chemistry, 286(52), 44620-44631 (2011)
Regulation of vascular endothelial growth factor (VEGF) splicing from pro-angiogenic to anti-angiogenic isoforms a novel therapeutic strategy for angiogenesis.
Nowak D G, et al.
The Journal of Biological Chemistry, 285(8), 5532-5540 (2010)
USP18 establishes the transcriptional and anti-proliferative interferon α/β differential.
Veronique F N, et al.
The Biochemical Journal, 446(3), 509-516 (2012)
A novel deletion of IGF1 in a patient with idiopathic short stature provides insight Into IGF1 haploinsufficiency.
Batey L, et al.
The Journal of Clinical Endocrinology and Metabolism, 99(1), E153-E159 (2014)
Articles
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Read More
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Read More
Related Content
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Read More
RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.