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DCTP-RO

Roche

dCTP

99% (dNTP), <0.9% (dNDP), solution, 100 mM

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Synonym(s):
2′-Deoxycytidine 5′-triphosphate disodium salt, dCTP-Na2
Empirical Formula (Hill Notation):
C9H14N3Na2O13P3
CAS Number:
Molecular Weight:
511.12
MDL number:
PubChem Substance ID:

description

PCR Grade, sodium salt

Quality Level

assay

99% (dNTP)

form

solution

usage

sufficient for 125,000 standard reactions (03732690001)
sufficient for 31,250 standard reactions (11969021001)
sufficient for 6,250 standard reactions (11934520001)

packaging

pkg of 1,250 μL (11969021001 [100 mM])
pkg of 4 × 1,250 μL (03732690001 [4x100 mM])
pkg of 250 μL (11934520001 [100 mM])

manufacturer/tradename

Roche

concentration

<0.9% (dNDP)
100 mM

storage temp.

−20°C

SMILES string

[Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2

InChI

1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1

InChI key

ABWVCNMFYVEBIB-CDNBRZBRSA-L

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This Item
11051458001DNTP-RODNSTP-RO
dCTP 99% (dNTP), &lt;0.9% (dNDP), solution, 100&#160;mM

DCTP-RO

dCTP

dCTP =98%, solution, 100&#160;mM, suitable for DNA sequencing, suitable for DNA amplification

11051458001

dCTP

Deoxynucleoside Triphosphate Set PCR Grade, sodium salt

DNTP-RO

Deoxynucleoside Triphosphate Set

Deoxynucleoside Triphosphate Set &#8804;1.5% (dNDP, HPLC), &#8805;=98% (dNTP), &#8804;0.5% (dNMP, HPLC)

DNSTP-RO

Deoxynucleoside Triphosphate Set

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

solution

form

solution

form

liquid

form

liquid

assay

99% (dNTP)

assay

98%

assay

99% (dNTP)

assay

≥98% (dntp)

concentration

<0.9% (dNDP), 100 mM

concentration

100 mM

concentration

<0.9% (dNDP)

concentration

-

packaging

pkg of 1,250 μL (11969021001 [100 mM]), pkg of 250 μL (11934520001 [100 mM]), pkg of 4 × 1,250 μL (03732690001 [4x100 mM])

packaging

pkg of 250 μL (25 μmol)

packaging

pkg of 4 × 1,250 μL (03622614001 [4x100 mM]), pkg of 4 × 250 μL (11969064001 [4x100 mM])

packaging

pkg of 4 × 100 μL (11277049001 [4x10 μmol]), pkg of 40 × 100 μL (11922505001 [40x100mM])

General description

dCTP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Application

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Quality

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Physical form

solution of dCTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Other Notes

For general laboratory use.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Alix T Coste et al.
Eukaryotic cell, 3(6), 1639-1652 (2004-12-14)
The ABC transporter genes CDR1 and CDR2 can be upregulated in Candida albicans developing resistance to azoles or can be upregulated by exposing cells transiently to drugs such as fluphenazine. The cis-acting drug-responsive element (DRE) present in the promoters of
Xiangang Zong et al.
Pflugers Archiv : European journal of physiology, 458(5), 891-899 (2009-06-27)
Second messenger-induced Ca(2+)-release from intracellular stores plays a key role in a multitude of physiological processes. In addition to 1,4,5-inositol trisphosphate (IP(3)), Ca(2+), and cyclic ADP ribose (cADPR) that trigger Ca(2+)-release from the endoplasmatic reticulum (ER), nicotinic acid adenine dinucleotide
Mahir Karababa et al.
Antimicrobial agents and chemotherapy, 48(8), 3064-3079 (2004-07-27)
Azole resistance in Candida albicans can be due to upregulation of multidrug transporters belonging to ABC (ATP-binding cassette) transporters (CDR1 and CDR2) or major facilitators (CaMDR1). Upregulation of these genes can also be achieved by exposure to fluphenazine, resulting in

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