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XTG360-RO

Roche

X-tremeGENE 360 Transfection Reagent

Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines

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Synonym(s):
transfection reagent

grade

for molecular biology

form

liquid (aqueous solution)

packaging

pkg of 0.4 mL (08724105001)
pkg of 1.0 mL (08724121001)
pkg of 5 × 1.0 mL (08724156001)

manufacturer/tradename

Roche

technique(s)

transfection: suitable

storage temp.

−20°C

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This Item
XTG9-ROSITRAN-RO70967
form

liquid (aqueous solution)

form

liquid (aqueous solution)

form

-

form

liquid

packaging

pkg of 0.4 mL (08724105001)

packaging

pkg of 0.4 mL (06365779001), pkg of 1.0 mL (06365787001), pkg of 5 × 1.0 mL (06365809001)

packaging

pkg of 1 mL (04476093001 [1 mg/ml]), pkg of 5 × 1 mL (04476115001 [5 x 1 mg/ml])

packaging

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Novagen®

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

X-tremeGENE 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility, X-tremeGENE 360 delivers high efficiency transfection in more cell types with low cytotoxicity.

Application

X-tremeGENE 360 Transfection Reagent is a high performance transfection reagent, free of animal-derived components.
  • Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2.
  • Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools.
  • Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex.
  • Suitable for transient and stable transfection.
  • Functions well in the presence or absence of serum.

Features and Benefits

X-tremeGENE 360 Transfection Reagent is a high-performing, versatile and reliable solution for hard-to-transfect and common cell types. As a proven and reliable reagent, X-tremeGENE 360 allows researchers to avoid guesswork when performing transfection. In addition, X-tremeGENE 360 exhibits:
  • Peak performance in a broad range of cell types
  • Suitable for multiple applications
  • Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components

Quality

Each lot of X-tremeGENE 360 Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE 360 Transfection Reagent (ratio 3:1 μL/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin. Number of Tests: Using the standard procedure, 1 mL of X-tremeGENE 360 Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

61.7 °F

Flash Point(C)

16.5 °C


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Articles

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques.

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Protocols

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids.

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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