07689

O′Meara′s Reagent

suitable for microbiology

• Synonym(s): Voges-Proskauer test with O’Meara Reagent
• UNSPSC Code: 41171621
• NACRES: NA.85

Properties

• product line: BioChemika
• Quality Level: 100
• shelf life: limited shelf life, expiry date on the label
• composition: creatine, 0.3 g ; dist. water, 100 mL ; potassium hydroxide, 40 g
• packaging: pkg of 100 mL
• storage condition: (Tightly closed. Dry.)
• technique(s): microbe id | metabolite detection: suitable
• application(s): environmental; food and beverages; microbiology
• suitability: Enterobacter spp.; Klebsiella spp.; Neisseria spp.; Pseudomonas spp.

Description

General description

O′Meara′s Reagent is used in the Voges-Proskauer (VP) test for the detection of acetoin production by bacterial strains. VP test is helpful in identifying members of the family Enterobacteriaceae based on their ability to produce acetylmethylcarbinol (AMC = acetonin) from glucose. Acetoin is an intermediate which is oxidized by oxygen under alkaline conditions (KOH) to 2,3-Butanediol. With creatinine (break down product from creatine) and the enhancement of α-naphtol a red color is formed (Meisenheimer complex)

Application

The O′Meara reagent is a component of the Voges-Proskauer (VP) test, which is a commonly used laboratory technique in industrial microbiology to differentiate between bacterial strains based on their ability to produce acetylmethylcarbinol (AMC) from glucose. The VP test is often used as a tool to identify and authenticate bacterial strains in the food, beverage, and pharmaceutical industries.

Analysis Note

To perform the VP test, a bacterial sample is inoculated into a nutrient broth and incubated. The O′Meara reagent, which contains a mixture of creatinine and potassium hydroxide (KOH), is then added to the culture. Acetoin produced in the medium by bacterial culture is oxidized under alkaline conditions in the presence of air to form diacetyl which reacts with creatine to give a pinkish-red compound.

Safety Information

• Pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H290,H302,H314
• pcodes: P234 - P270 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
• Storage Class: 8B - Non-combustible corrosive hazardous materials
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter