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39441

Millipore

Nitrate Reagent B

suitable for microbiology

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Synonym(s):
Sulfanilic acid solution, 4-Aminobenzenesulfonic acid
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.85

shelf life

limited shelf life, expiry date on the label

Quality Level

composition

acetic acid (30%), 1000 mL
sulfanilic acid, 8.0 g

technique(s)

microbe id | metabolite detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical

microbiology

suitability

Enterobacter spp.
Escherichia coli
Neisseria spp.
anaerobic bacteria
coliforms

SMILES string

Nc1ccc(cc1)S(O)(=O)=O

InChI

1S/C6H7NO3S/c7-5-1-3-6(4-2-5)11(8,9)10/h1-4H,7H2,(H,8,9,10)

InChI key

HVBSAKJJOYLTQU-UHFFFAOYSA-N

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1 of 4

This Item
2519178.223381.00686
Nitrate Reagent B suitable for microbiology

Millipore

39441

Nitrate Reagent B

Sulfanilic acid ACS reagent, 99%

Sigma-Aldrich

251917

Sulfanilic acid

Sulfanilic acid for synthesis

Sigma-Aldrich

8.22338

Sulfanilic acid

Sulfanilic acid GR for analysis ACS,Reag. Ph Eur

Supelco

1.00686

Sulfanilic acid

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

composition

acetic acid (30%), 1000 mL

composition

-

composition

-

composition

-

technique(s)

microbe id | metabolite detection: suitable

technique(s)

-

technique(s)

-

technique(s)

-

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical

application(s)

-

application(s)

-

application(s)

-

suitability

Enterobacter spp.

suitability

-

suitability

-

suitability

-

Application

α-Naphthylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphthylamine to form a red azo dye.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Sulfanilic acid for synthesis

Sigma-Aldrich

8.22338

Sulfanilic acid

1-Naphthylamine analytical standard

Supelco

34390

1-Naphthylamine

1-Naphthylamine 97%

Sigma-Aldrich

N9005

1-Naphthylamine

Nitrate Test photometric, 0.3-30.0 mg/L (NO3-N), Spectroquant®

Supelco

1.01842

Nitrate Test

Sulfanilic acid ACS reagent, 99%

Sigma-Aldrich

251917

Sulfanilic acid

Han Ming Gan et al.
Chemosphere, 82(4), 507-513 (2010-11-26)
A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100 mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation
Han Ming Gan et al.
Microbiology (Reading, England), 158(Pt 8), 1933-1941 (2012-05-23)
The gene coding for the oxygenase component, sadA, of 4-aminobenzenesulfonate (4-ABS) 3,4-dioxygenase in Hydrogenophaga sp. PBC was previously identified via transposon mutagenesis. Expression of wild-type sadA in trans restored the ability of the sadA mutant to grow on 4-ABS. The
Kwan Kim et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 100, 10-14 (2012-02-14)
We estimated the apparent size of the 'hot site' for surface-enhanced Raman scattering (SERS) located within the gaps between Pt nanoparticles and a flat Ag substrate. Initially, no Raman peaks were detected for 4-aminobenzenethiol (4-ABT) on a flat Ag substrate.
Han Ming Gan et al.
FEMS microbiology letters, 318(2), 108-114 (2011-02-18)
Genes involved in the 4-aminobenzenesulfonate (4-ABS) degradation pathway of Hydrogenophaga sp. PBC were identified using transposon mutagenesis. The screening of 10,000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. Genes with insertions that impaired the ability
Kwan Kim et al.
The journal of physical chemistry. B, 109(40), 18929-18934 (2006-07-21)
Raman scattering measurements were conducted for 4-aminobenzenethiol (4-ABT) assembled on powdered copper substrates. Initially, very weak Raman peaks were detected, but upon attaching Ag nanoparticles probably via NH2 groups onto 4-ABT/Cu, distinct Raman spectra were observed. Considering the fact that

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