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25455

4-Chloro-7-nitrobenzofurazan

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Synonym(s):

4-Chloro-7-nitro-1,2,3-benzoxadiazole, NBD-chloride

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About This Item

Empirical Formula (Hill Notation):
C6H2ClN3O3
CAS Number:
10199-89-0
Molecular Weight:
199.55
Beilstein/REAXYS Number:
614212
EC Number:
233-496-4
MDL number:
MFCD00005808
UNSPSC Code:
12352100
PubChem Substance ID:
329752738
NACRES:
NA.32

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product line

BioReagent

assay

≥97.0% (HPLC)

mp

97-100 °C
97-99 °C (lit.)

fluorescence

λex 336 nm in methanol
λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)

suitability

suitable for fluorescence

SMILES string

[O-][N+](=O)c1ccc(Cl)c2nonc12

InChI

1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

Inchi Key

IGHBXJSNZCFXNK-UHFFFAOYSA-N

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1 of 4

This Item
726397163260M5760
4-Chloro-7-nitrobenzofurazan BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Sigma-Aldrich

25455

4-Chloro-7-nitrobenzofurazan

4-(Chloromethyl)benzonitrile ≥97.0%

Sigma-Aldrich

726397

4-(Chloromethyl)benzonitrile

4-Chloro-7-nitrobenzofurazan 98%

Sigma-Aldrich

163260

4-Chloro-7-nitrobenzofurazan

4-(4-Methoxybenzylamino)-7-nitrobenzofurazan

Sigma-Aldrich

M5760

4-(4-Methoxybenzylamino)-7-nitrobenzofurazan

assay

≥97.0% (HPLC)

assay

≥97.0% (HPLC), 97.0-103.0% (T)

assay

98%

assay

>97.5% (HPLC)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

mp

97-100 °C, 97-99 °C (lit.)

mp

-

mp

97-99 °C (lit.)

mp

-

product line

BioReagent

product line

-

product line

-

product line

-

fluorescence

λex 336 nm in methanol

fluorescence

-

fluorescence

-

fluorescence

-

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

Application

4-Chloro-7-nitrobenzofurazan (NBD-chloride) is a fluorescence assay reagent used to label free sulfhydryls and N-terminals within proteins. NBD-chloride is used as an enzyme inhibitor and as a trapping agent for cysteine sulfenic acid.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8958
BCCN3021
BCCL6618
BCCJ9973
BCCJ0887

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Leslie B Poole et al.
Methods in enzymology, 348, 122-136 (2002-03-12)
C165S AhpC in its sulfenate (Cys-SO-) and presumed thiolate (Cys-S-) forms at pH 7 (pKa for sulfenic acid about pH 6.1) exhibit low extinction absorbance bands around 367 and 324 nm, respectively. Sulfenic acid content of the protein can be
P D Bragg et al.
Biochimica et biophysica acta, 1413(3), 159-171 (1999-11-11)
Pyridine nucleotide transhydrogenases of bacterial cytosolic membranes and mitochondrial inner membranes are proton pumps in which hydride transfer between NADP(+) and NAD(+) is coupled to proton translocation across cytosolic or mitochondrial membranes. The pyridine nucleotide transhydrogenase of Escherichia coli is
Rob van Zwieten et al.
Biochimica et biophysica acta, 1821(12), 1493-1500 (2012-09-11)
Maintenance of the asymmetric distribution of phospholipids across the plasma membrane is a prerequisite for the survival of erythrocytes. Various stimuli have been shown to induce scrambling of phospholipids and thereby exposure of phosphatidylserine (PS). In two types of patients
Emmanuel C Gorospe et al.
Gastrointestinal endoscopy, 76(5), 933-938 (2012-09-18)
There are currently 2 existing confocal laser endomicroscopy (CLE) platforms: probe-based CLE (pCLE) and endoscope-based CLE (eCLE) systems, each with its own criteria for identifying dysplasia in Barrett's esophagus (BE). The diagnostic performance of these 2 systems has not been
Elham Raeisi et al.
The Journal of membrane biology, 245(10), 633-642 (2012-08-14)
This study investigated whether molecules spontaneously transported inside cells, like glucose derivatives, can also be used as electropermeabilization markers. Uptake of a fluorescent deoxyglucose derivative (2-NBDG) by normal and electropermeabilized cells in culture was analyzed. 2-NBDG was added to DC-3F
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