Mixture A is prepared by adding 5ml of the Epoxy Embedding Medium with 8 ml of DDSA. Mix well for at least 20 minutes. Mixture B is prepared by adding 8ml of Epoxy Embedding Medium with 7ml of NMA. Immediately before use mix 13 ml of Mixture A with 15 ml of Mixture B. Carefully add 16 drops of DPM-30 (1.5% to 2.0%). The DPM-30 (accelerator) should be added carefully since the block can become discolored if not measured properly and the block may be too brittle for satisfactory sectioning.
Please refer to the usage protocol here:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/433/080/45359dat.pdf
45359
Epoxy Embedding Medium kit
embedding resin for electron microscopy
Synonym(s):
Epon™ substitute embedding medium kit
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Product Name
Epoxy Embedding Medium kit, embedding resin for electron microscopy
pH
5-7
density
1.9 g/cm3
application(s)
hematology
histology
storage temp.
2-8°C
Quality Level
1 of 4
This Item | 45345 | 45346 | 45347 |
|---|---|---|---|
| pH 5-7 | pH - | pH - | pH - |
| density 1.9 g/cm3 | density - | density 1.00 g/mL at 20 °C | density 1.232 g/mL at 25 °C (lit.) |
| application(s) hematology | application(s) hematology | application(s) agriculture | application(s) hematology |
| storage temp. 2-8°C | storage temp. room temp | storage temp. - | storage temp. room temp |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Application
Epoxy Embedding Medium Kit has been used in the following applications:
- to study the role of NDRG2, an essential colonic epithelial barrier regulator, in gut homeostasis maintenance and colitis-associated tumor development[1]
- to study the interaction of polymer scaffold nanofibers with growing cells[2]
- to study the morphological characterization of postembryonic development of blood–spleen barrier in ducks[3]
- to understand the role of NOD1/NOD2 receptors in Fusobacterium nucleatum-mediated neutrophil extracellular traps[4]
Features and Benefits
- Excellent medium for embedding a wide variety of tissues, including both plant and animal.
- Facilitates rapid embedding (less than 3 hours).
- Low viscosity facilitates rapid tissue penetration.
- Desirable hardness is easy to obtain to suit a specific tissue type.
General description
The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).
Other Notes
45345 Epoxy Embedding Medium 2x250 ml
45347 Hardener MNA 250 ml
45346 Hardener DDSA 250 ml
45348 Accelerator DMP 30 250 ml
45347 Hardener MNA 250 ml
45346 Hardener DDSA 250 ml
45348 Accelerator DMP 30 250 ml
Legal Information
Epon is a trademark of Hexion, Inc.
related product
Product No.
Description
Pricing
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
275.0 °F
flash_point_c
135 °C
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A Sawaguchi et al.
Journal of microscopy, 234(2), 113-117 (2009-04-29)
The goal of specimen preparation for transmission electron microscopy is to obtain high-quality ultra-thin sections with which we can correlate cellular structure to physiological function. In this study, we newly developed a capsule-supporting ring that can be useful for resin
Investigation of the effects of semaphorin 3A on new bone formation in a rat calvarial defect model.
Sevinç Kenan et al.
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 47(3), 473-483 (2019-01-09)
This study investigates the effects of semaphorin 3A on new bone formation in an experimental rat model. Cortical bone defects, 5 mm, were created in the calvaria of 40 Wistar rats, which were then separated into three groups: empty defect (control)
High resolution 3D microscopy study of cardiomyocytes on polymer scaffold nanofibers reveals formation of unusual sheathed structure
Balashov, et al.
Acta Biomaterialia (2018)
Role of NOD1/NOD2 receptors in Fusobacterium nucleatum mediated NETosis
Alyami, et al.
Microbial Pathogenesis (2019)
C K E Bleck et al.
Journal of microscopy, 237(1), 23-38 (2010-01-09)
Bacteria are generally difficult specimens to prepare for conventional resin section electron microscopy and mycobacteria, with their thick and complex cell envelope layers being especially prone to artefacts. Here we made a systematic comparison of different methods for preparing Mycobacterium
Related Content
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Should I first prepare Mixture A: and separately Mixture B: and then use these mixtures to infiltrate the tissue in ratios 1:1 and 1:2, or should I mix all four components together at once and then perform the infiltration? I am not clear about this step
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Hallo, gelten nach dem Aushärten die gleichen H- und P-Sätze wie für das Kit?
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After curing, the hazard and precautionary statements will still apply to this kit.
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