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45359

Sigma-Aldrich

Epoxy Embedding Medium kit

Synonym(s):
Epon substitute embedding medium kit
NACRES:
NA.47

Quality Level

pH

5-7

density

1.9 g/cm3

application(s)

hematology
histology

storage temp.

2-8°C

General description

Polymerization 35 °, 45 °, 60 °C for 24 h each, alternatively 60 °C for 12 h.
The components can also be ordered separately under the product numbers.

Application

Epoxy Embedding Medium (Epon_812 substitute) is a very widely used embedding resin for electron microscopy, as it penetrates into the tissue specimen faster than Araldite and other polymers due to its low viscosity. Epoxy can be hardened easely and uniformly at low temperatures by the addition of DDSA, NMA and the accelerator DPM-30. Slight shrinkage does occur during curing. Epoxy embedding medium is useful for embedding a variety of tissues as a wide hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and NMA). Universal embedding medium for semi- and ultrathin sections.

Components

45345 Epoxy Embedding Medium 2x250 ml
45347 Hardener MNA 250 ml
45346 Hardener DDSA 250 ml
45348 Accelerator DMP 30 250 ml

Legal Information

Epon is a trademark of Hexion, Inc.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

275.0 °F

Flash Point(C)

135 °C

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Quotes and Ordering

A Sawaguchi et al.
Journal of microscopy, 234(2), 113-117 (2009-04-29)
The goal of specimen preparation for transmission electron microscopy is to obtain high-quality ultra-thin sections with which we can correlate cellular structure to physiological function. In this study, we newly developed a capsule-supporting ring that can be useful for resin
Sevinç Kenan et al.
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 47(3), 473-483 (2019-01-09)
This study investigates the effects of semaphorin 3A on new bone formation in an experimental rat model. Cortical bone defects, 5 mm, were created in the calvaria of 40 Wistar rats, which were then separated into three groups: empty defect (control)
Konstantin E Mochalov et al.
Ultramicroscopy, 182, 118-123 (2017-07-04)
In the past decade correlative microscopy, which combines the potentials of different types of high-resolution microscopies with a variety of optical microspectroscopy techniques, has been attracting increasing attention in material science and biological research. One of outstanding solutions in this
Emma Cantisani et al.
The Analyst, 144(7), 2375-2386 (2019-02-26)
Red stains are a common discolouration on stone cultural heritage all over the world. These are very difficult to remove and little is known about the reddish pigmentation. Numerous red stains were mapped on the Baptistery of San Giovanni in
Kunio Nagashima et al.
Methods in molecular biology (Clifton, N.J.), 697, 83-91 (2010-12-01)
This chapter outlines the procedures for ex vivo TEM preparation of nanoparticle-containing tissue or cell culture samples using an epoxy resin embedding method. The purpose of this procedure is to preserve the structure of tissue in a hardened epoxy block

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