53404

SAFC

Atto 465 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

product line

BioReagent

assay

≥90% (HPLC)
≥90% (degree of coupling)

manufacturer/tradename

ATTO-TEC GmbH

λ

in acetonitrile (with 0.1% acetic acid)

UV absorption

λ: 454-460 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OCl(=O)(=O)=O.Nc1ccc2C=C3C=CC(=N)C=C3N(CCCC(=O)ON4C(=O)CCC4=O)c2c1

InChI

1S/C21H20N4O4.ClHO4/c22-15-5-3-13-10-14-4-6-16(23)12-18(14)24(17(13)11-15)9-1-2-21(28)29-25-19(26)7-8-20(25)27;2-1(3,4)5/h3-6,10-12,22H,1-2,7-9,23H2;(H,2,3,4,5)

InChI key

NROCSLJOXCQUHR-UHFFFAOYSA-N

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

David J Posson et al.
Nature, 436(7052), 848-851 (2005-08-12)
Voltage-gated ion channels open and close in response to voltage changes across electrically excitable cell membranes. Voltage-gated potassium (Kv) channels are homotetramers with each subunit constructed from six transmembrane segments, S1-S6 (ref. 2). The voltage-sensing domain (segments S1-S4) contains charged...
Radek Macháň et al.
Analytical and bioanalytical chemistry, 399(10), 3547-3554 (2011-02-05)
Z-scan fluorescence correlation spectroscopy (FCS) is employed to characterize the interaction between arenicin-1 and supported lipid bilayers (SLBs) of different compositions. Lipid analogue C8-BODIPY 500/510C5-HPC and ATTO 465 labelled arenicin-1 are used to detect changes in lipid and peptide diffusion...
Andriy Chmyrov et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 7(11), 1378-1385 (2008-10-30)
Photo-induced switching of dyes into dark, long-lived states, such as a triplet state, has recently gained increasing interest, as a means to achieve ultra-high optical resolution. Additionally, these long lived states are often highly environment-sensitive and their photodynamics can thus...
Gerald Donnert et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(31), 11440-11445 (2006-07-26)
We demonstrate far-field fluorescence microscopy with a focal-plane resolution of 15-20 nm in biological samples. The 10- to 12-fold multilateral increase in resolution below the diffraction barrier has been enabled by the elimination of molecular triplet state excitation as a...
Waldemar Waldeck et al.
International journal of medical sciences, 8(2), 97-105 (2011-02-01)
Fluorescent proteins (FPs) are established tools for new applications, not-restricted to the cell biological research. They could also be ideal in surgery enhancing the precision to differentiate between the target tissue and the surrounding healthy tissue. FPs like the KillerRed...
Articles
Fluorescent Labeling of Peptides
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