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A5304

Sigma-Aldrich

Agarose

Special High EEO

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Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

form

powder

technique(s)

electrophoresis: suitable
immunoelectrophoresis: suitable

impurities

≤11% moisture content

ash

≤1.5%

turbidity

≤4 NTU (1.5% gel)

EEO

≤0.40

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

gel strength

≥700 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.2%

storage temp.

room temp

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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1 of 4

This Item
A4679A9539A2929
vibrant-m

A5304

Agarose

-
vibrant-m

A4679

Agarose

-
vibrant-m

A9539

Agarose

Essential+ Grade
vibrant-m

A2929

Agarose

-
form

powder

form

powder

form

powder

form

powder

EEO

≤0.40

EEO

0.09-0.13

EEO

0.09-0.13

EEO

≤0.10

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

transition temp

gel point 34-38 °C (1.5% gel)

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

transition temp

gel point 38-43 °C (10% gel)

technique(s)

electrophoresis: suitable, immunoelectrophoresis: suitable

technique(s)

electrophoresis: suitable, immunodiffusion: suitable, immunoelectrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

Suitability

Suitable for counterimmunoelectrophoresis and immunoelectrophoretic techniques with significant cathodal migration.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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