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B950-05

Sigma-Aldrich

Bovine Renal Artery Smooth Muscle Cells: BRASMC (Cryovial)

NACRES:
NA.81

Quality Level

biological source

bovine renal artery (normal, tunica intima and media)

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 60

morphology

smooth muscle

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

renal disease; cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Bovine Renal Artery Smooth Muscle Cells (BRASMC) are derived from tunica intima and tunica media of normal bovine renal arteries. They are cryopreserved at second passage and can be cultured and propagated at least 12 population doublings. Studies show cyclosporine-induced renal artery smooth muscle contraction is associated with increases in the phosphorylation of specific contractile regulatory proteins1. The renal artery smooth muscle is uniquely refractory to angiotensin stimulation and may be protective in the role of the kidney in regulating blood pressure through the renin-angiotensin system2.

Beall, A. et al, Biochem Biophys Acta., 1449(1):41-9 (1999)
Whitney, E.G. et al, J. Surg. Res. 61(2):307-10 (1996)

Products are for research use only. They are not intended for human, animal, or diagnostic application.
Animal tissues were USDA inspected.

Cell Line Origin

Artery

Application

blood flow to kidneys

Components

Bovine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Bovine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 12 doublings

Subculture Routine

Please refer to the BRASMC Culture Protocol.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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